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Nucleic Acids Research Pages 1950-1955  

Fast and simple purification of chemically modified hammerhead ribozymes using a lipophilic capture tag
   Diisopropyl(DL-[alpha]-tocopheryloxy)chlorosilane (1)
   1-[Diisopropyl(DL-[alpha]-tocopheryloxy)silyl]oxy-6-hydroxy-hexane (2)
   1-[Diisopropyl(DL-[alpha]-tocopheryloxy)silyl]oxy-6-(2-cyano-ethoxyN,N-diisopropylaminophosphinoxy)hexane (3)
Acknowledgements
References

Fast and simple purification of chemically modified hammerhead ribozymes using a lipophilic capture tag

Fast and simple purification of chemically modified hammerhead ribozymes using a lipophilic capture tag

Brian S. Sproat*, Thomas Rupp, Norbert Menhardt, Doreen Keane and Barbro Beijer

Innovir GmbH, Olenhuser Landstrasse 20b, D-37124 Rosdorf, Germany

Received as resubmission December 7, 1998; Revised and Accepted February 15, 1999

ABSTRACT

A new type of 5[prime]-lipophilic capture tag is described, enabling the facile reverse phase HPLC purification of chemically modified hammerhead ribozymes (oligozymes) whilst still carrying the 2[prime]-O-tert.-butyldi-methylsilyl protection of the essential riboses. In its most convenient form, the capture tag consists of a simple diol, such as hexan-1,6-diol, which at one end is attached via a silyl residue to a highly lipophilic entity such as . . . [Full Text of this Article]

Diisopropyl(DL-[alpha]-tocopheryloxy)chlorosilane (1)

1-[Diisopropyl(DL-[alpha]-tocopheryloxy)silyl]oxy-6-hydroxy-hexane (2)

1-[Diisopropyl(DL-[alpha]-tocopheryloxy)silyl]oxy-6-(2-cyano-ethoxyN,N-diisopropylaminophosphinoxy)hexane (3)

ACKNOWLEDGEMENTS

REFERENCES


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