Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (819K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (47)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Sherburne, C. K.
Right arrow Articles by Taylor, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sherburne, C. K.
Right arrow Articles by Taylor, D. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2000, Vol. 28, No. 10 2177-2186
© 2000 Oxford University Press

The complete DNA sequence and analysis of R27, a large IncHI plasmid from Salmonella typhi that is temperature sensitive for transfer

Craig K. Sherburne1, Trevor D. Lawley2, Matthew W. Gilmour1, F. R. Blattner3, Valerie Burland3, Erik Grotbeck3, Debra J. Rose3 and Diane E. Taylor1,2,*

1Department of Medical Microbiology and Immunology and 2Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada and 3Laboratory of Genetics, University of Wisconsin, Madison, WI, USA

Salmonella typhi, the causative agent of typhoid fever, annually infects 16 million people and kills 600 000 world wide. Plasmid-encoded multiple drug resistance in S.typhi is always encoded by plasmids of incompatibility group H (IncH). The complete DNA sequence of the large temperature-sensitive conjugative plasmid R27, the prototype for the IncHI1 family of plasmids, has been compiled and analyzed. This 180 kb plasmid contains 210 open reading frames (ORFs), of which 14 have been previously identified and 56 exhibit similarity to other plasmid and prokaryotic ORFs. A number of insertion elements were found, including the full Tn10 transposon, which carries tetracycline resistance genes. Two transfer regions, Tra1 and Tra2, are present, which are separated by a minimum of 64 kb. Homologs of the DNA-binding proteins TlpA and H-NS that act as temperature-regulated repressors in other systems have been located in R27. Sequence analysis of transfer and replication regions supports a mosaic-like structure for R27. The genes responsible for conjugation and plasmid maintenance have been identified and mechanisms responsible for thermosensitive transfer are discussed.

* To whom correspondence should be addressed at: Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada. Tel: +1 780 492 4777; Fax: +1 780 492 7521; Email: diane.taylor@ualberta.ca


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
M.-D. Phan, C. Kidgell, S. Nair, K. E. Holt, A. K. Turner, J. Hinds, P. Butcher, F. J. Cooke, N. R. Thomson, R. Titball, et al.
Variation in Salmonella enterica Serovar Typhi IncHI1 Plasmids during the Global Spread of Resistant Typhoid Fever
Antimicrob. Agents Chemother., February 1, 2009; 53(2): 716 - 727.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
M. Bielaszewska, R. Prager, L. Vandivinit, A. Musken, A. Mellmann, N. J. Holt, P. I. Tarr, H. Karch, and W. Zhang
Detection and Characterization of the Fimbrial sfp Cluster in Enterohemorrhagic Escherichia coli O165:H25/NM Isolates from Humans and Cattle
Appl. Envir. Microbiol., January 1, 2009; 75(1): 64 - 71.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
M.-J. Kim, I. Hirono, K. Kurokawa, T. Maki, J. Hawke, H. Kondo, M. D. Santos, and T. Aoki
Complete DNA Sequence and Analysis of the Transferable Multiple-Drug Resistance Plasmids (R Plasmids) from Photobacterium damselae subsp. piscicida Isolates Collected in Japan and the United States
Antimicrob. Agents Chemother., February 1, 2008; 52(2): 606 - 611.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K. E. Holt, N. R. Thomson, J. Wain, M. D. Phan, S. Nair, R. Hasan, Z. A. Bhutta, M. A. Quail, H. Norbertczak, D. Walker, et al.
Multidrug-Resistant Salmonella enterica Serovar Paratyphi A Harbors IncHI1 Plasmids Similar to Those Found in Serovar Typhi
J. Bacteriol., June 1, 2007; 189(11): 4257 - 4264.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
J. E. Gunton, M. W. Gilmour, K. P. Baptista, T. D. Lawley, and D. E. Taylor
Interaction between the co-inherited TraG coupling protein and the TraJ membrane-associated protein of the H-plasmid conjugative DNA transfer system resembles chromosomal DNA translocases
Microbiology, February 1, 2007; 153(2): 428 - 441.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
M. Doyle, M. Fookes, A. Ivens, M. W. Mangan, J. Wain, and C. J. Dorman
An H-NS-like Stealth Protein Aids Horizontal DNA Transmission in Bacteria
Science, January 12, 2007; 315(5809): 251 - 252.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
T. J. Johnson, Y. M. Wannemeuhler, J. A. Scaccianoce, S. J. Johnson, and L. K. Nolan
Complete DNA Sequence, Comparative Genomics, and Prevalence of an IncHI2 Plasmid Occurring among Extraintestinal Pathogenic Escherichia coli Isolates
Antimicrob. Agents Chemother., November 1, 2006; 50(11): 3929 - 3933.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. Doyle and C. J. Dorman
Reciprocal Transcriptional and Posttranscriptional Growth-Phase-Dependent Expression of sfh, a Gene That Encodes a Paralogue of the Nucleoid-Associated Protein H-NS
J. Bacteriol., November 1, 2006; 188(21): 7581 - 7591.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
T. C. Elton, S. J. Holland, L. S. Frost, and B. Hazes
F-Like Type IV Secretion Systems Encode Proteins with Thioredoxin Folds That Are Putative DsbC Homologues
J. Bacteriol., December 15, 2005; 187(24): 8267 - 8277.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
J. E. Gunton, M. W. Gilmour, G. Alonso, and D. E. Taylor
Subcellular localization and functional domains of the coupling protein, TraG, from IncHI1 plasmid R27
Microbiology, November 1, 2005; 151(11): 3549 - 3561.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
G. Alonso, K. Baptista, T. Ngo, and D. E. Taylor
Transcriptional organization of the temperature-sensitive transfer system from the IncHI1 plasmid R27
Microbiology, November 1, 2005; 151(11): 3563 - 3573.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
C. Neylon, A. V. Kralicek, T. M. Hill, and N. E. Dixon
Replication Termination in Escherichia coli: Structure and Antihelicase Activity of the Tus-Ter Complex
Microbiol. Mol. Biol. Rev., September 1, 2005; 69(3): 501 - 526.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
N. Forns, R. C. Banos, C. Balsalobre, A. Juarez, and C. Madrid
Temperature-Dependent Conjugative Transfer of R27: Role of Chromosome- and Plasmid-Encoded Hha and H-NS Proteins
J. Bacteriol., June 15, 2005; 187(12): 3950 - 3959.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. W. Gilmour and D. E. Taylor
A Subassembly of R27-Encoded Transfer Proteins Is Dependent on TrhC Nucleoside Triphosphate-Binding Motifs for Function but Not Formation
J. Bacteriol., March 15, 2004; 186(6): 1606 - 1613.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
J. A. Lewis, A. R. Horswill, B. E. Schwem, and J. C. Escalante-Semerena
The Tricarballylate Utilization (tcuRABC) Genes of Salmonella enterica Serovar Typhimurium LT2
J. Bacteriol., March 15, 2004; 186(6): 1629 - 1637.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
M. R. Mulvey, D. Boyd, L.-K. Ng, S. Brown, M. Lombos, B. Ciebin, A. Li, F. Jamieson, and P. Stuart
First Canadian Salmonella enterica Serovar Typhi Isolate Harboring an Integron
Antimicrob. Agents Chemother., February 1, 2004; 48(2): 689 - 690.
[Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
J. Wain, L. T. Diem Nga, C. Kidgell, K. James, S. Fortune, T. Song Diep, T. Ali, P. O Gaora, C. Parry, J. Parkhill, et al.
Molecular Analysis of incHI1 Antimicrobial Resistance Plasmids from Salmonella Serovar Typhi Strains Associated with Typhoid Fever
Antimicrob. Agents Chemother., September 1, 2003; 47(9): 2732 - 2739.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
T. D. Lawley and D. E. Taylor
Characterization of the Double-Partitioning Modules of R27: Correlating Plasmid Stability with Plasmid Localization
J. Bacteriol., May 15, 2003; 185(10): 3060 - 3067.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Wei, M. B. Goldberg, V. Burland, M. M. Venkatesan, W. Deng, G. Fournier, G. F. Mayhew, G. Plunkett III, D. J. Rose, A. Darling, et al.
Complete Genome Sequence and Comparative Genomics of Shigella flexneri Serotype 2a Strain 2457T
Infect. Immun., May 1, 2003; 71(5): 2775 - 2786.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
T. D. Lawley, M. W. Gilmour, J. E. Gunton, D. M. Tracz, and D. E. Taylor
Functional and Mutational Analysis of Conjugative Transfer Region 2 (Tra2) from the IncHI1 Plasmid R27
J. Bacteriol., January 15, 2003; 185(2): 581 - 591.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
C. M. Parry, T. T. Hien, G. Dougan, N. J. White, and J. J. Farrar
Typhoid Fever
N. Engl. J. Med., November 28, 2002; 347(22): 1770 - 1782.
[Full Text] [PDF]

Home page
 J. Bacteriol.Home page
D. Boltner, C. MacMahon, J. T. Pembroke, P. Strike, and A. M. Osborn
R391: a Conjugative Integrating Mosaic Comprised of Phage, Plasmid, and Transposon Elements
J. Bacteriol., September 15, 2002; 184(18): 5158 - 5169.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
J. W. Beaber, B. Hochhut, and M. K. Waldor
Genomic and Functional Analyses of SXT, an Integrating Antibiotic Resistance Gene Transfer Element Derived from Vibrio cholerae
J. Bacteriol., August 1, 2002; 184(15): 4259 - 4269.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
T. Murata, M. Ohnishi, T. Ara, J. Kaneko, C.-G. Han, Y. F. Li, K. Takashima, H. Nojima, K. Nakayama, A. Kaji, et al.
Complete Nucleotide Sequence of Plasmid Rts1: Implications for Evolution of Large Plasmid Genomes
J. Bacteriol., June 15, 2002; 184(12): 3194 - 3202.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
T. D. Lawley, M. W. Gilmour, J. E. Gunton, L. J. Standeven, and D. E. Taylor
Functional and Mutational Analysis of Conjugative Transfer Region 1 (Tra1) from the IncHI1 Plasmid R27
J. Bacteriol., April 15, 2002; 184(8): 2173 - 2180.
[Abstract] [Full Text]

Home page
 Antimicrob. Agents Chemother.Home page
C. Kehrenberg and S. Schwarz
Molecular Analysis of Tetracycline Resistance in Pasteurella aerogenes
Antimicrob. Agents Chemother., October 1, 2001; 45(10): 2885 - 2890.
[Abstract] [Full Text]

Home page
 J. Bacteriol.Home page
B. Martinez, J. Tomkins, L. P. Wackett, R. Wing, and M. J. Sadowsky
Complete Nucleotide Sequence and Organization of the Atrazine Catabolic Plasmid pADP-1 from Pseudomonas sp. Strain ADP
J. Bacteriol., October 1, 2001; 183(19): 5684 - 5697.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
D. Boyd, G. A. Peters, A. Cloeckaert, K. S. Boumedine, E. Chaslus-Dancla, H. Imberechts, and M. R. Mulvey
Complete Nucleotide Sequence of a 43-Kilobase Genomic Island Associated with the Multidrug Resistance Region of Salmonella enterica Serovar Typhimurium DT104 and Its Identification in Phage Type DT120 and Serovar Agona
J. Bacteriol., October 1, 2001; 183(19): 5725 - 5732.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. B. Prentice, K. D. James, J. Parkhill, S. G. Baker, K. Stevens, M. N. Simmonds, K. L. Mungall, C. Churcher, P. C. F. Oyston, R. W. Titball, et al.
Yersinia pestis pFra Shows Biovar-Specific Differences and Recent Common Ancestry with a Salmonella enterica Serovar Typhi Plasmid
J. Bacteriol., April 15, 2001; 183(8): 2586 - 2594.
[Abstract] [Full Text]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.