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Nucleic Acids Research, 2000, Vol. 28, No. 10 E46-e46
© 2000 Oxford University Press

Alkaline fixation drastically improves the signal of in situ hybridization

Eugenia Basyuk*, Edouard Bertrand1 and Laurent Journot

UPR 9023 CNRS, Mecanismes Moleculaires des Communications Cellulaires, CCIPE, 141 rue de la Cardonille, 34094 Montpellier Cedex 05, France and 1UMR 5535 CNRS, Institut de Genetique Moleculaire de Montpellier, 1919 route de Mende, 34293 Montpellier Cedex 05, France

In situ hybridization (ISH) is widely used to detect DNA and RNA sequences within the cell and tissue sections. The important step in performing this technique is tissue fixation. We investigated the influence of the pH of the fixative on the outcome of ISH. Our studies indicate that alkaline formaldehyde dramatically increases the ISH signal with RNA probes. The increase in signal was observed for detection of low as well as for high abundance messages. The sensitivity of the method was increased 5- to 6-fold.

* To whom correspondence should be addressed. Tel: +33 467 14 29 63; Fax: +33 467 54 24 32; Email: basyuk@ccipe.montp.inserm.fr


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