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Nucleic Acids Research, 2000, Vol. 28, No. 11 2214-2220
© 2000 Oxford University Press

Isolation of an essential Schizosaccharomyces pombe gene, prp31+, that links splicing and meiosis

Danielle T. Bishop1,2, W. Hayes McDonald3, Kathleen L. Gould3 and Susan L. Forsburg1,*

1Molecular Biology and Virology Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA, 2Department of Biology, University of California, San Diego, CA 92093, USA and 3Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, TN 37233, USA

We carried out a screen for mutants that arrest prior to premeiotic S phase. One of the strains we isolated contains a temperature-sensitive allele mutation in the fission yeast prp31+ gene. The prp31-E1 mutant is defective in vegetative cell growth and in meiotic progression. It is synthetically lethal with prp6 and displays a pre-mRNA splicing defect at the restrictive temperature. We cloned the wild-type gene by complementation of the temperature-sensitive mutant phenotype. Prp31p is closely related to human and budding yeast PRP31 homologs and is likely to function as a general splicing factor in both vegetative growth and sexual differentiation.

* To whom correspondence should be addressed. Tel: +1 858 453 4100; Fax: +1 858 457 4765; Email: forsburg@salk.edu


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