Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (340K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (28)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Gongora, C.
Right arrow Articles by Mechti, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gongora, C.
Right arrow Articles by Mechti, N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2000, Vol. 28, No. 12 2333-2341
© 2000 Oxford University Press

A unique ISRE, in the TATA-less human Isg20 promoter, confers IRF-1-mediated responsiveness to both interferon type I and type II

Celine Gongora, Genevieve Degols, Lucile Espert, The Duc Hua and Nadir Mechti*

INSERM Unité U475, 99 rue Puech Villa, 34197 Montpellier cedex 5, France

Interferons (IFNs) encode a family of secreted proteins involved in a number of regulatory functions such as control of cell proliferation, differentiation and regulation of the immune system. Their diverse biological actions are thought to be mediated by the products of specific but usually overlapping sets of cellular genes induced in the target cells. We have recently isolated a human cDNA encoding a new nuclear bodies-associated protein (PML-NBs), which we have termed Isg20. In this report, we describe the cloning and functional characterization of the Isg20 promoter region and the identification of sequence elements and trans-acting factors implicated in its regulation. In the absence of any recognizable TATA or CAAT elements, Isg20 promoter basal activity is dependent upon the positive transcription factors Sp-1 and USF-1. Interestingly, we demonstrate that a unique interferon stimulated response element (ISRE) mediates both IFN type I and type II Isg20 induction in the absence of functional {gamma}-activated sequence. These inductions are strictly dependent upon of the IFN regulatory factor 1 (IRF-1). In addition, we show that the ISRE is also implicated in the constitutive transcriptional activity of Isg20 gene.

* To whom correspondence should be addressed. Tel: +33 4 67 63 62 71; Fax: +33 4 67 04 18 63; Email: mechti@u475.montp.inserm.fr


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
H. Kaku and T. L. Rothstein
Fas Apoptosis Inhibitory Molecule Expression in B Cells Is Regulated through IRF4 in a Feed-Forward Mechanism
J. Immunol., November 1, 2009; 183(9): 5575 - 5581.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
O. Tliba and Y. Amrani
Airway Smooth Muscle Cell as an Inflammatory Cell: Lessons Learned from Interferon Signaling Pathways
Proceedings of the ATS, January 1, 2008; 5(1): 106 - 112.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Eckert, S. E. M. Meek, and K. L. Ball
A Novel Repressor Domain Is Required for Maximal Growth Inhibition by the IRF-1 Tumor Suppressor
J. Biol. Chem., August 11, 2006; 281(32): 23092 - 23102.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
L. Espert, G. Degols, Y.-L. Lin, T. Vincent, M. Benkirane, and N. Mechti
Interferon-induced exonuclease ISG20 exhibits an antiviral activity against human immunodeficiency virus type 1
J. Gen. Virol., August 1, 2005; 86(8): 2221 - 2229.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. C. Kuo and K. L. Calame
B Lymphocyte-Induced Maturation Protein (Blimp)-1, IFN Regulatory Factor (IRF)-1, and IRF-2 Can Bind to the Same Regulatory Sites
J. Immunol., November 1, 2004; 173(9): 5556 - 5563.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Ruiz-Ruiz, C. Ruiz de Almodovar, A. Rodriguez, G. Ortiz-Ferron, J. M. Redondo, and A. Lopez-Rivas
The Up-regulation of Human Caspase-8 by Interferon-{gamma} in Breast Tumor Cells Requires the Induction and Action of the Transcription Factor Interferon Regulatory Factor-1
J. Biol. Chem., May 7, 2004; 279(19): 19712 - 19720.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
S. P. PERSENGIEV, X. ZHU, and M. R. GREEN
Nonspecific, concentration-dependent stimulation and repression of mammalian gene expression by small interfering RNAs (siRNAs)
RNA, January 1, 2004; 10(1): 12 - 18.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
D. Chaussabel, R. T. Semnani, M. A. McDowell, D. Sacks, A. Sher, and T. B. Nutman
Unique gene expression profiles of human macrophages and dendritic cells to phylogenetically distinct parasites
Blood, July 15, 2003; 102(2): 672 - 681.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Espert, G. Degols, C. Gongora, D. Blondel, B. R. Williams, R. H. Silverman, and N. Mechti
ISG20, a New Interferon-induced RNase Specific for Single-stranded RNA, Defines an Alternative Antiviral Pathway against RNA Genomic Viruses
J. Biol. Chem., April 25, 2003; 278(18): 16151 - 16158.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.