Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (189K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Angelastro, J. M.
Right arrow Articles by Vitolo, O. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Angelastro, J. M.
Right arrow Articles by Vitolo, O. V.
Related Collections
Right arrow Miscellaneous/other
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2000, Vol. 28, No. 12 E62-e62
© 2000 Oxford University Press

Improved NlaIII digestion of PAGE-purified 102 bp ditags by addition of a single purification step in both the SAGE and microSAGE protocols

James M. Angelastro*, Lars P. Klimaschewski and Ottavio V. Vitolo

Department of Pathology and Taub Center for Alzheimer’s Disease Research and Center for Neurobiology and Behavior, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA

Despite the success of microarray technologies, serial analysis of gene expression (SAGE) still remains the only technique that allows an accurate quantitative and qualitative analysis of cell transcription in a variety of physiological and pathological conditions. Nevertheless, the efficiency of SAGE is limited by the numerous gel purification steps required and these increase the possibility of contamination and reduce or inhibit the activity of the enzymes used in the protocol. In order to eliminate this problem, we have modified the original protocol by adding a single purification step before NlaIII digestion of the ditags. This allows us to increase the yield of digested ditags without reducing the amount of DNA or affecting the subsequent concatemerization.

* To whom correspondence should be addressed. Tel: +1 212 305 6370; Fax: +1 212 305 5498; Email: jma14@columbia.edu


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
A. P. So, R. F. B. Turner, and C. A. Haynes
Increasing the efficiency of SAGE adaptor ligation bydirected ligation chemistry
Nucleic Acids Res., July 6, 2004; 32(12): e96 - e96.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E. H. Margulies, S. L. R. Kardia, and J. W. Innis
Identification and prevention of a GC content bias in SAGE libraries
Nucleic Acids Res., June 15, 2001; 29(12): e60 - e60.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. M. Angelastro, L. Klimaschewski, S. Tang, O. V. Vitolo, T. A. Weissman, L. T. Donlin, M. L. Shelanski, and L. A. Greene
Identification of diverse nerve growth factor-regulated genes by serial analysis of gene expression (SAGE) profiling
PNAS, September 12, 2000; 97(19): 10424 - 10429.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.