Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (258K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (12)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Suzuki-Ishigaki, S.
Right arrow Articles by Ohtsu, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suzuki-Ishigaki, S.
Right arrow Articles by Ohtsu, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2000, Vol. 28, No. 14 2627-2633
© 2000 Oxford University Press

The mouse L-histidine decarboxylase gene: structure and transcriptional regulation by CpG methylation in the promoter region

Satsuki Suzuki-Ishigaki, Keiko Numayama-Tsuruta, Atsuo Kuramasu, Eiko Sakurai, Yoko Makabe, Sanae Shimura1, Kunio Shirato1, Kazuhiko Igarashi2, Takehiko Watanabe and Hiroshi Ohtsu*

Department of Cellular Pharmacology, Tohoku University School of Medicine, Seiryo-machi 2-1, Aoba-ku, Sendai 980-8575, Japan, 1Department of Internal Medicine I, Tohoku University School of Medicine, Seiryo-machi 1-1, Aoba-ku, Sendai 980-8574, Japan and 2Department of Biochemistry, Hiroshima University School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8551, Japan

To investigate the regulation of mouse L-histidine decarboxylase (HDC) gene expression, we isolated genomic DNA clones encoding HDC. Structural analysis revealed that the mouse HDC gene was composed of 12 exons, spanning ~24 kb. Northern blotting analysis indicated that, among the cell lines examined, a high level of HDC gene expression was restricted to mature mast cell lines and an erythroblastic cell line. The gene was induced strongly in the mouse immature mast cell line P815 after incubation in the peritoneal cavity of BDF1 mice. We observed that the promoter region was demethylated in the HDC-expressing cell lines and in induced P815 cells. Interestingly, forced demethylation by 5-azacytidine (5-azaC) treatment induced high expression of HDC mRNA in P815 cells. The activity of a mouse HDC promoter–reporter construct stably transfected in P815 cells was repressed by in vitro patch-methylation. This low promoter activity of the patch-methylated reporter construct was restored after 5-azaC treatment, which demethylated the patch-methylated promoter. These results indicate that DNA methylation state of the promoter region controls HDC gene expression.

* To whom correspondence should be addressed. Tel: +81 22 717 8058; Fax: +81 22 717 8058; Email: ohtsu@mail.cc.tohoku.ac.jp


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
W. Ai, Y. Liu, M. Langlois, and T. C. Wang
Kruppel-like Factor 4 (KLF4) Represses Histidine Decarboxylase Gene Expression through an Upstream Sp1 Site and Downstream Gastrin Responsive Elements
J. Biol. Chem., March 5, 2004; 279(10): 8684 - 8693.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. M. Thomas, K. Haleem, A. B. Siddique, W. J. Simmons, N. Sen, D.-J. Zhang, and V. K. Tsiagbe
Regulation of Mouse Mammary Tumor Virus env Transcriptional Activator Initiated Mammary Tumor Virus Superantigen Transcripts in Lymphomas of SJL/J Mice: Role of Ikaros, Demethylation, and Chromatin Structural Change in the Transcriptional Activation of Mammary Tumor Virus Superantigen
J. Immunol., January 1, 2003; 170(1): 218 - 227.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.