Nucleic Acids Research, 2000, Vol. 28, No. 14 2709-2716
© 2000 Oxford University Press
Dmc1 of Schizosaccharomyces pombe plays a role in meiotic recombination
Department of Molecular Genetics, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita City, Osaka 565-0871, Japan
We report here a Schizosaccharomyces pombe gene (dmc1+) that resembles budding yeast DMC1 in the region immediately upstream of the rad24+ gene. We showed by northern and Southern blot analysis that dmc1+ and rad24+ are co-transcribed as a bicistronic mRNA of 2.8 kb with meiotic specificity, whereas rad24+ itself is constitutively transcribed as a 1.0-kb mRNA species during meiosis. Induction of the bicistronic transcript is under the control of a meiosis-specific transcription factor, Ste11. Disruption of both dmc1+ and rad24+ had no effect on mitosis or spore formation, and dmc1
cells displayed no change in sensitivity to UV or 
irradiation relative to the wild type. Tetrad analysis indicated that Dmc1 is involved in meiotic recombination. Analysis of gene conversion frequencies using single and double mutants of dmc1 and rhp51 indicated that both Dmc1 and Rhp51 function in meiotic gene conversion. These observations, together with a high level of sequence identity, indicate that the dmc1+ gene of S.pombe is a structural homolog of budding yeast DMC1, sharing both similar and distinct functions in meiosis.
* To whom correspondence should be addressed. Tel: +81 6 6875 3980; Fax: +81 6 6875 5192; Email: hnojima@biken.osaka-u.ac.jp Present address: Yoshimi Tanaka, ICRF Clare Hall Laboratories, South Mimms EN6 3LD, UK
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