Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (327K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (24)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Fukushima, K.
Right arrow Articles by Nojima, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fukushima, K.
Right arrow Articles by Nojima, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2000, Vol. 28, No. 14 2709-2716
© 2000 Oxford University Press

Dmc1 of Schizosaccharomyces pombe plays a role in meiotic recombination

Kentaro Fukushima, Yoshimi Tanaka, Kentaro Nabeshima, Takahiro Yoneki, Takahiro Tougan, Seiji Tanaka and Hiroshi Nojima*

Department of Molecular Genetics, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita City, Osaka 565-0871, Japan

We report here a Schizosaccharomyces pombe gene (dmc1+) that resembles budding yeast DMC1 in the region immediately upstream of the rad24+ gene. We showed by northern and Southern blot analysis that dmc1+ and rad24+ are co-transcribed as a bicistronic mRNA of 2.8 kb with meiotic specificity, whereas rad24+ itself is constitutively transcribed as a 1.0-kb mRNA species during meiosis. Induction of the bicistronic transcript is under the control of a meiosis-specific transcription factor, Ste11. Disruption of both dmc1+ and rad24+ had no effect on mitosis or spore formation, and dmc1{Delta} cells displayed no change in sensitivity to UV or {gamma} irradiation relative to the wild type. Tetrad analysis indicated that Dmc1 is involved in meiotic recombination. Analysis of gene conversion frequencies using single and double mutants of dmc1 and rhp51 indicated that both Dmc1 and Rhp51 function in meiotic gene conversion. These observations, together with a high level of sequence identity, indicate that the dmc1+ gene of S.pombe is a structural homolog of budding yeast DMC1, sharing both similar and distinct functions in meiosis.

* To whom correspondence should be addressed. Tel: +81 6 6875 3980; Fax: +81 6 6875 5192; Email: hnojima@biken.osaka-u.ac.jp Present address: Yoshimi Tanaka, ICRF Clare Hall Laboratories, South Mimms EN6 3LD, UK


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J. Hikiba, K. Hirota, W. Kagawa, S. Ikawa, T. Kinebuchi, I. Sakane, Y. Takizawa, S. Yokoyama, B. Mandon-Pepin, A. Nicolas, et al.
Structural and functional analyses of the DMC1-M200V polymorphism found in the human population
Nucleic Acids Res., July 1, 2008; 36(12): 4181 - 4190.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
G. Kutty, B. Hernandez-Novoa, M. Czapiga, and J. A. Kovacs
Pneumocystis Encodes a Functional S-Adenosylmethionine Synthetase Gene
Eukaryot. Cell, February 1, 2008; 7(2): 258 - 267.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. A. Farah, G. Cromie, L. Davis, W. W. Steiner, and G. R. Smith
Activation of an Alternative, Rec12 (Spo11)-Independent Pathway of Fission Yeast Meiotic Recombination in the Absence of a DNA Flap Endonuclease
Genetics, December 1, 2005; 171(4): 1499 - 1511.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Sauvageau, A. Z. Stasiak, I. Banville, M. Ploquin, A. Stasiak, and J.-Y. Masson
Fission Yeast Rad51 and Dmc1, Two Efficient DNA Recombinases Forming Helical Nucleoprotein Filaments
Mol. Cell. Biol., June 1, 2005; 25(11): 4377 - 4387.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. C. Hope, M. Maftahi, and G. A. Freyer
A Postsynaptic Role for Rhp55/57 That Is Responsible for Cell Death in {Delta}rqh1 Mutants Following Replication Arrest in Schizosaccharomyces pombe
Genetics, June 1, 2005; 170(2): 519 - 531.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. T. Saito, T. Tougan, D. Okuzaki, T. Kasama, and H. Nojima
Mcp6, a meiosis-specific coiled-coil protein of Schizosaccharomyces pombe, localizes to the spindle pole body and is required for horsetail movement and recombination
J. Cell Sci., January 15, 2005; 118(2): 447 - 459.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
C. Ellermeier, H. Schmidt, and G. R. Smith
Swi5 Acts in Meiotic DNA Joint Molecule Formation in Schizosaccharomyces pombe
Genetics, December 1, 2004; 168(4): 1891 - 1898.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. A. Young, R. W. Hyppa, and G. R. Smith
Conserved and Nonconserved Proteins for Meiotic DNA Breakage and Repair in Yeasts
Genetics, June 1, 2004; 167(2): 593 - 605.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. L. Grishchuk and J. Kohli
Five RecA-like Proteins of Schizosaccharomyces pombe Are Involved in Meiotic Recombination
Genetics, November 1, 2003; 165(3): 1031 - 1043.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. G. Catlett and S. L. Forsburg
Schizosaccharomyces pombe Rdh54 (TID1) Acts with Rhp54 (RAD54) to Repair Meiotic Double-Strand Breaks
Mol. Biol. Cell, November 1, 2003; 14(11): 4707 - 4720.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. Okuzaki, W. Satake, A. Hirata, and H. Nojima
Fission yeast meu14+ is required for proper nuclear division and accurate forespore membrane formation during meiosis II
J. Cell Sci., July 1, 2003; 116(13): 2721 - 2735.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
U. Markmann-Mulisch, M. Z. Hadi, K. Koepchen, J. C. Alonso, V. E. A. Russo, J. Schell, and B. Reiss
The organization of Physcomitrella patensRAD51 genes is unique among eukaryotic organisms
PNAS, March 5, 2002; 99(5): 2959 - 2964.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Davis and G. R. Smith
Meiotic recombination and chromosome segregation in Schizosaccharomyces pombe
PNAS, July 17, 2001; 98(15): 8395 - 8402.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. Watanabe, K. Miyashita, T. T. Saito, T. Yoneki, Y. Kakihara, K. Nabeshima, Y. A. Kishi, C. Shimoda, and H. Nojima
Comprehensive isolation of meiosis-specific genes identifies novel proteins and unusual non-coding transcripts in Schizosaccharomyces pombe
Nucleic Acids Res., June 1, 2001; 29(11): 2327 - 2337.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.