Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (1010K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (20)
Right arrowRequest Permissions
Citing Articles
Right arrowScopus Links
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lechner, M. S.
Right arrow Articles by Dressler, G. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lechner, M. S.
Right arrow Articles by Dressler, G. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2000, Vol. 28, No. 14 2741-2751
© 2000 Oxford University Press

PTIP, a novel BRCT domain-containing protein interacts with Pax2 and is associated with active chromatin

Mark S. Lechner, Inna Levitan and Gregory R. Dressler*

Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA

The Pax gene family encodes transcription factors essential for organ and tissue development in higher eukaryotes. Pax proteins are modular with an N-terminal DNA binding domain, a C-terminal transcription activation domain, and a transcription repression domain called the octapeptide. How these domains interact with the cellular machinery remains unclear. In this report, we describe the isolation and characterization of a novel gene and its encoded protein, PTIP, which binds to the activation domain of Pax2 and other Pax proteins. PTIP binds to Pax2 in vitro, in the yeast two-hybrid assay and in tissue culture cells. The binding of PTIP to Pax2 is inhibited by the octapeptide repression domain. The PTIP protein contains five BRCT domains, first identified in BRCA1 and other nuclear proteins involved in DNA repair/recombination or cell cycle control. Pax2 and PTIP co-localize in the cell nucleus to actively expressed chromatin and the nuclear matrix fraction. For the first time, these results point to a link between Pax transcription factors and active chromatin.

* To whom correspondence should be addressed at: MSRB 1, Room 4510, 1150 West Medical Center Drive, Ann Arbor, MI 48109-0650, USA. Tel: +1 734 764 6490; Fax: +1 734 763 6640; Email: dressler@umich.edu Present address: Mark S. Lechner, The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J. Wu, M. J. Prindle, G. R. Dressler, and X. Yu
PTIP Regulates 53BP1 and SMC1 at the DNA Damage Sites
J. Biol. Chem., July 3, 2009; 284(27): 18078 - 18084.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. Fang, H. Ren, J. Liu, K. M. Cadigan, S. R. Patel, and G. R. Dressler
Drosophila ptip is essential for anterior/posterior patterning in development and interacts with the PcG and trxG pathways
Development, June 1, 2009; 136(11): 1929 - 1938.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. K. Pandita and C. Richardson
Chromatin remodeling finds its place in the DNA double-strand break response
Nucleic Acids Res., April 1, 2009; 37(5): 1363 - 1377.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Gong, Y.-W. Cho, J.-E. Kim, K. Ge, and J. Chen
Accumulation of Pax2 Transactivation Domain Interaction Protein (PTIP) at Sites of DNA Breaks via RNF8-dependent Pathway Is Required for Cell Survival after DNA Damage
J. Biol. Chem., March 13, 2009; 284(11): 7284 - 7293.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
G. R. Dressler
Epigenetics, Development, and the Kidney
J. Am. Soc. Nephrol., November 1, 2008; 19(11): 2060 - 2067.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
W. Mu, W. Wang, and J. C. Schimenti
An Allelic Series Uncovers Novel Roles of the BRCT Domain-Containing Protein PTIP in Mouse Embryonic Vascular Development
Mol. Cell. Biol., October 15, 2008; 28(20): 6439 - 6451.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
I. M. Munoz, P. A. Jowsey, R. Toth, and J. Rouse
Phospho-epitope binding by the BRCT domains of hPTIP controls multiple aspects of the cellular response to DNA damage
Nucleic Acids Res., August 13, 2007; 35(16): 5312 - 5322.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-W. Cho, T. Hong, S. Hong, H. Guo, H. Yu, D. Kim, T. Guszczynski, G. R. Dressler, T. D. Copeland, M. Kalkum, et al.
PTIP Associates with MLL3- and MLL4-containing Histone H3 Lysine 4 Methyltransferase Complex
J. Biol. Chem., July 13, 2007; 282(28): 20395 - 20406.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
R. Kopan, H.-T. Cheng, and K. Surendran
Molecular Insights into Segmentation along the Proximal Distal Axis of the Nephron
J. Am. Soc. Nephrol., July 1, 2007; 18(7): 2014 - 2020.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
D. Kim, M. Wang, Q. Cai, H. Brooks, and G. R. Dressler
Pax Transactivation-Domain Interacting Protein Is Required for Urine Concentration and Osmotolerance in Collecting Duct Epithelia
J. Am. Soc. Nephrol., May 1, 2007; 18(5): 1458 - 1465.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
I. Issaeva, Y. Zonis, T. Rozovskaia, K. Orlovsky, C. M. Croce, T. Nakamura, A. Mazo, L. Eisenbach, and E. Canaani
Knockdown of ALR (MLL2) Reveals ALR Target Genes and Leads to Alterations in Cell Adhesion and Growth
Mol. Cell. Biol., March 1, 2007; 27(5): 1889 - 1903.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. A. Jowsey, A. J. Doherty, and J. Rouse
Human PTIP Facilitates ATM-mediated Activation of p53 and Promotes Cellular Resistance to Ionizing Radiation
J. Biol. Chem., December 31, 2004; 279(53): 55562 - 55569.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
S.-L. Zhang, B. Moini, and J. R. Ingelfinger
Angiotensin II Increases Pax-2 Expression in Fetal Kidney Cells Via the AT2 Receptor
J. Am. Soc. Nephrol., June 1, 2004; 15(6): 1452 - 1465.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
I. A. Manke, D. M. Lowery, A. Nguyen, and M. B. Yaffe
BRCT Repeats As Phosphopeptide-Binding Modules Involved in Protein Targeting
Science, October 24, 2003; 302(5645): 636 - 639.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
E. A. Cho, M. J. Prindle, and G. R. Dressler
BRCT Domain-Containing Protein PTIP Is Essential for Progression through Mitosis
Mol. Cell. Biol., March 1, 2003; 23(5): 1666 - 1673.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. F. Revers, J. M. Cardone, D. Bonatto, J. Saffi, M. Grey, H. Feldmann, M. Brendel, and J. A. P. Henriques
Thermoconditional modulation of the pleiotropic sensitivity phenotype by the Saccharomyces cerevisiae PRP19 mutant allele pso4-1
Nucleic Acids Res., November 15, 2002; 30(22): 4993 - 5003.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Hoffmeister, A. Ropolo, S. Vasseur, G. V. Mallo, H. Bodeker, B. Ritz-Laser, G. R. Dressler, M. I. Vaccaro, J.-C. Dagorn, S. Moreno, et al.
The HMG-I/Y-related Protein p8 Binds to p300 and Pax2 trans-Activation Domain-interacting Protein to Regulate the trans-Activation Activity of the Pax2A and Pax2B Transcription Factors on the Glucagon Gene Promoter
J. Biol. Chem., June 14, 2002; 277(25): 22314 - 22319.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. V. Emelyanov, C. R. Kovac, M. A. Sepulveda, and B. K. Birshtein
The Interaction of Pax5 (BSAP) with Daxx Can Result in Transcriptional Activation in B Cells
J. Biol. Chem., March 22, 2002; 277(13): 11156 - 11164.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Shimizu, P.-Y. Bourillot, S. J. Nielsen, A. M. Zorn, and J. B. Gurdon
Swift Is a Novel BRCT Domain Coactivator of Smad2 in Transforming Growth Factor {beta} Signaling
Mol. Cell. Biol., June 15, 2001; 21(12): 3901 - 3912.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.