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Nucleic Acids Research, 2000, Vol. 28, No. 14 E71-e71
© 2000 Oxford University Press

Silanized nucleic acids: a general platform for DNA immobilization

Anil Kumar, Ola Larsson, Daniela Parodi and Zicai Liang*

Genomics Technology Unit, Center for Genomics Research, Karolinska Institutet, Theorells vägen 3, 17177, Stockholm, Sweden

We have developed a method for simultaneous deposition and covalent cross-linking of oligonucleotide or PCR products on unmodified glass surfaces. By covalently conjugating an active silyl moiety onto oligonucleotides or cDNA in solutions we have generated a new class of modified nucleic acids, namely silanized nucleic acids. Such silanized molecules can be immobilized instantly onto glass surfaces after manual or automated deposition. This method provides a simple and rapid, yet very efficient, solution to the immobilization of prefabricated oligonucleotides and DNA for chip production.

* To whom correspondence should be addressed. Tel: +46 8 7286371; Fax: +46 8 331547; Email: zicai.liang@cgr.ki.se


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