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Nucleic Acids Research, 2000, Vol. 28, No. 16 3017-3021
© 2000 Oxford University Press

Base J originally found in Kinetoplastida is also a minor constituent of nuclear DNA of Euglena gracilis

Dennis Dooijes, Inês Chaves, Rudo Kieft, Anita Dirks-Mulder, William Martin1 and Piet Borst*

Division of Molecular Biology and Centre for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands and 1Institute of Genetics, Technical University of Braunschweig, Spielmannstrasse 7, 38023 Braunschweig, Germany

We have analyzed DNA of Euglena gracilis for the presence of the unusual minor base ß-D-glucosyl-hydroxymethyluracil or J, thus far only found in kinetoplastid flagellates and in Diplonema. Using antibodies specific for J and post-labeling of DNA digests followed by two-dimensional thin-layer chromatography of labeled nucleotides, we show that ~0.2 mole percent of Euglena DNA consists of J, an amount similar to that found in DNA of Trypanosoma brucei. By staining permeabilized Euglena cells with anti-J antibodies, we show that J is rather uniformly distributed in the Euglena nucleus, and does not co-localize to a substantial extent with (GGGTTA)n repeats, the putative telomeric repeats of Euglena. Hence, most of J in Euglena appears to be non-telomeric. Our results add to the existing evidence for a close phylogenetic relation between kinetoplastids and euglenids.

* To whom correspondence should be addressed. Tel: +31 20 512 2880; Fax: +31 20 669 1383; Email: pborst@nki.nlPresent addresses:Dennis Dooijes, Department of Clinical Genetics, Erasmus University Rotterdam, PO Box 1738, 3000 DR Rotterdam, The NetherlandsInês Chaves, Cell Biology and Genetics, Erasmus University Rotterdam, PO Box 1738, 3000 DR Rotterdam, The NetherlandsWilliam Martin, Institute of Ecological Plant Physiology, Heinrich-Heine Universität Düsseldorf, 40225 Düsseldorf, Germany


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