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Nucleic Acids Research, 2000, Vol. 28, No. 16 3125-3133
© 2000 Oxford University Press

The precursor strategy: terminus methoxyoxalamido modifiers for single and multiple functionalization of oligodeoxyribonucleotides

Nikolai N. Polushin*

Fidelity Systems Inc., 7961 Cessna Avenue, Gaithersburg, MD 20879, USA

Synthesis of new terminus modifiers, bearing, along with a phosphoramidite moiety, one, two or four methoxyoxalamido (MOX) precursor groups, is described. These modifiers are introduced onto the 5'-end of a synthetic oligodeoxyribonucleotide as the last step of an automated synthesis to form the MOX precursor oligonucleotide. The MOX groups are then post-synthetically derivatized with an appropriate primary amine to construct a 5'-modified oligonucleotide. The efficiency and simplicity of the novel modifying strategy were demonstrated in the synthesis of a number of 5'-functionalized oligonucleotides.

* Tel: +1 301 527 0804; Fax: +1 301 527 8250; Email: npolouchine@fidelitysystems.com


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