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Nucleic Acids Research, 2000, Vol. 28, No. 16 E76-e76
© 2000 Oxford University Press

Efficient purification of DNA fragments using a protein binding membrane

Øistein Ihle1,* and Terje E. Michaelsen1,2

1National Institute of Public Health, Department of VAIM, PO Box 4404 Torshov, 0462 Oslo, Norway and 2Department of Pharmacognosy, Institute of Pharmacy, University of Oslo, Norway

A novel and efficient method has been developed for isolation of correctly digested DNA fragments without the use of classic size-dependent electrophoretic separation methods. To achieve this, DNA fragments are end-labelled by haptens. After specific endonuclease digestion of the hapten-labelled DNA, the DNA is incubated with a protein that specifically binds to the hapten. The incubation mixture is then passed through a cartridge containing a protein-binding membrane that does not bind DNA. Undigested and partly digested DNA are retained on the membrane, while correctly digested DNA is selectively recovered for use in further downstream applications.

* To whom correspondence should be addressed. Tel: +47 22 042515; Fax: +47 22353605; Email: oistein.ihle@folkehelsa.no


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