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Nucleic Acids Research, 2000, Vol. 28, No. 2 424-429
© 2000 Oxford University Press

APOBEC-1 dependent cytidine to uridine editing of apolipoprotein B RNA in yeast

Geoffrey S. C. Dance1, Mark P. Sowden1,2, Yan Yang2 and Harold C. Smith1,2,3,*

1Department of Biochemistry and Biophysics, 2Department of Pathology and 3The Environmental Health Sciences Center, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA

Cytidine to uridine editing of apolipoprotein B (apoB) mRNA requires the cytidine deaminase APOBEC-1 as well as a tripartite sequence motif flanking a target cytidine in apoB mRNA and an undefined number of auxiliary proteins that mediate RNA recognition and determine site-specific editing. Yeast engineered to express APOBEC-1 and apoB mRNA supported editing under conditions of late log phase growth and stationary phase. The cis-acting sequence requirements and the intracellular distribution of APOBEC-1 in yeast were similar to those described in mammalian cells. These findings suggest that auxiliary protein functions necessary for the assembly of editing complexes, or ‘editosomes’, are expressed in yeast and that the distribution of editing activity is to the cell nucleus.

* To whom correspondence should be addressed at: Department of Biochemistry and Biophysics, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA. Tel: +1 716 275 4267; Fax: +1 716 273 1027; Email: harold_smith@urmc.rochester.edu


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