Nucleic Acids Research, 2000, Vol. 28, No. 2 552-559
© 2000 Oxford University Press
Bacillus subtilis LrpC is a sequence-independent DNA-binding and DNA-bending protein which bridges DNA
Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Campus Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain
Genetic evidence suggests that the Bacillus subtilis lrpC gene product participates in cell growth and sporulation. The purified LrpC protein, which has a predicted molecular mass of 16.4 kDa, is a tetramer in solution. LrpC binds with higher affinity (Kapp ~ 80 nM) to intrinsically curved DNA than to non-curved DNA (Kapp ~ 700 nM). DNase I footprinting and the supercoiling of relaxed circular plasmid DNA in the presence of topoisomerase I revealed that LrpC induces DNA bending and constrains DNA supercoils in vitro. The LrpC protein cooperatively increases DNA binding of the bona fide DNA-binding and DNA-bending protein Hbsu. LrpC forms inter- and intramolecular bridges on linear and supercoiled DNA molecules, resulting in a large network and DNA compactation. Collectively, these findings suggest that LrpC is an architectural protein and that its activities could provide a means to modulate DNA transactions.
* To whom correspondence should be addressed. Tel: +34 91585 4528; Fax: +34 91585 4506; Email: sayora@cnb.uam.es Present address: Angels Tapias, Departamento de Genética y Microbiología, Universitat Autónoma de Barcelona, Bellaterra, 08193 Barcelona, Spain
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Liu, L. Guo, R. Guo, R. L. Wong, H. Hernandez, J. Hu, Y. Chu, I. J. Amster, W. B. Whitman, and L. Huang The Sac10b Homolog in Methanococcus maripaludis Binds DNA at Specific Sites J. Bacteriol., April 1, 2009; 191(7): 2315 - 2329. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Shrivastava and R. Ramachandran Mechanistic insights from the crystal structures of a feast/famine regulatory protein from Mycobacterium tuberculosis H37Rv Nucleic Acids Res., December 18, 2007; 35(21): 7324 - 7335. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-H. Chan and J. T. Y. Wong Concentration-dependent organization of DNA by the dinoflagellate histone-like protein HCc3 Nucleic Acids Res., April 4, 2007; (2007) gkm165v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Thaw, S. E. Sedelnikova, T. Muranova, S. Wiese, S. Ayora, J. C. Alonso, A. B. Brinkman, J. Akerboom, J. van der Oost, and J. B. Rafferty Structural insight into gene transcriptional regulation and effector binding by the Lrp/AsnC family Nucleic Acids Res., March 9, 2006; 34(5): 1439 - 1449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Lopez-Torrejon, M. I. Martinez-Jimenez, and S. Ayora Role of LrpC from Bacillus subtilis in DNA transactions during DNA repair and recombination Nucleic Acids Res., January 10, 2006; 34(1): 120 - 129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Gonzalez-Huici, M. Alcorlo, M. Salas, and J. M. Hermoso Phage {phi}29 Proteins p1 and p17 Are Required for Efficient Binding of Architectural Protein p6 to Viral DNA In Vivo J. Bacteriol., December 15, 2004; 186(24): 8401 - 8406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Gonzalez-Huici, M. Alcorlo, M. Salas, and J. M. Hermoso Binding of phage {Phi}29 architectural protein p6 to the viral genome: evidence for topological restriction of the phage linear DNA Nucleic Acids Res., July 1, 2004; 32(11): 3493 - 3502. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Gonzalez-Huici, M. Salas, and J. M. Hermoso Genome wide, supercoiling-dependent in vivo binding of a viral protein involved in DNA replication and transcriptional control Nucleic Acids Res., April 26, 2004; 32(8): 2306 - 2314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Beloin, J. Jeusset, B. Revet, G. Mirambeau, F. Le Hegarat, and E. Le Cam Contribution of DNA Conformation and Topology in Right-handed DNA Wrapping by the Bacillus subtilis LrpC Protein J. Biol. Chem., February 7, 2003; 278(7): 5333 - 5342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Pavlov, D. I. Cherny, I. V. Nazimov, A. I. Slesarev, and V. Subramaniam Identification, cloning and characterization of a new DNA-binding protein from the hyperthermophilic methanogen Methanopyrus kandleri Nucleic Acids Res., February 1, 2002; 30(3): 685 - 694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. T. Dame, C. Wyman, and N. Goosen H-NS mediated compaction of DNA visualised by atomic force microscopy Nucleic Acids Res., September 15, 2000; 28(18): 3504 - 3510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Beloin, R. Exley, A.-L. Mahé, M. Zouine, S. Cubasch, and F. Le Hégarat Characterization of LrpC DNA-Binding Properties and Regulation of Bacillus subtilis lrpC Gene Expression J. Bacteriol., August 15, 2000; 182(16): 4414 - 4424. [Abstract] [Full Text]
|
|
|
|
|
|
J. Enoru-Eta, D. Gigot, T.-L. Thia-Toong, N. Glansdorff, and D. Charlier Purification and Characterization of Sa-Lrp, a DNA-Binding Protein from the Extreme Thermoacidophilic Archaeon Sulfolobus acidocaldarius Homologous to the Bacterial Global Transcriptional Regulator Lrp J. Bacteriol., July 1, 2000; 182(13): 3661 - 3672. [Abstract] [Full Text]
|
|
|
|
|
|
A. Napoli, M. Kvaratskelia, M. F. White, M. Rossi, and M. Ciaramella A Novel Member of the Bacterial-Archaeal Regulator Family Is a Nonspecific DNA-binding Protein and Induces Positive Supercoiling J. Biol. Chem., March 30, 2001; 276(14): 10745 - 10752. [Abstract] [Full Text] [PDF]
|
|