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Nucleic Acids Research, 2000, Vol. 28, No. 2 E7-e7
© 2000 Oxford University Press

Interspersed repetitive sequence (IRS)–PCR for typing of whole genome radiation hybrid panels

Heinz Himmelbauer*, Leonard C. Schalkwyk and Hans Lehrach

Max-Planck-Institute of Molecular Genetics, Ihnestraße 73, D-14195 Berlin-Dahlem, Germany

The typing of a radiation hybrid (RH) panel is generally achieved using a unique primer pair for each marker. We here describe a complementing approach utilizing IRS–PCR. Advantages of this technology include the use of a single universal primer to specify any locus, the rapid typing of RH lines by hybridization, and the conservative use of hybrid DNA. The technology allows the mapping of a clone without the requirement for STS generation. To test the technique, we have mapped 48 BAC clones derived from mouse chromosome 12 which we mostly identified using complex probes. As mammalian genomes are repeat-rich, the technology can easily be adapted to species other than mouse.

* To whom correspondence should be addressed. Tel: +49 30 8413 1354; Fax: +49 30 8413 1128; Email: himmelbauer@molgen.mpg.de


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