Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (1148K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (17)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Read, D.
Right arrow Articles by Kornberg, T. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Read, D.
Right arrow Articles by Kornberg, T. B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2000, Vol. 28, No. 20 3864-3870
© 2000 Oxford University Press

Functional studies of the BTB domain in the Drosophila GAGA and Mod(mdg4) proteins

Doug Read, Manish J. Butte1, Abby F. Dernburg, Manfred Frasch2 and Thomas B. Kornberg*

Department of Biochemistry and Biophysics and 1Graduate Group in Biophysics, University of California, San Francisco, CA 94143, USA and 2Department of Biochemistry and Molecular Biology, Mount Sinai School of Medicine, New York, NY 10029, USA

The BTB/POZ (BTB) domain is an approximately 120 residue sequence that is conserved at the N-terminus of many proteins in both vertebrates and invertebrates. We found that the protein encoded by a lethal allele of the Drosophila modifier of mdg4 [mod(mdg4)] gene has two mutated residues in its BTB domain. The identities of the residues at the positions of these mutations are highly conserved in the BTB domain family of proteins, and when the corresponding mutations were engineered into the BTB domain-containing GAGA protein, the activity of GAGA as a transcription activator in a transient transfection assay was severely reduced. The functional equivalence of the BTB domains was established by showing that the BTB domain of the mod(mdg4) protein can effectively substitute for that of GAGA.

* To whom correspondence should be addressed. Tel: +1 415 476 8821; Fax: +1 415 514 1470; Email: tkornberg@biochem.ucsf.edu Present addresses: Doug Read, Amersham/Pharmacia Biotech, 654 Minnesota Street, San Francisco, CA 94107, USA Abby F. Dernburg, Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GeneticsHome page
M. Soltani-Bejnood, S. E. Thomas, L. Villeneuve, K. Schwartz, C.-s. Hong, and B. D. McKee
Role of the mod(mdg4) Common Region in Homolog Segregation in Drosophila Male Meiosis
Genetics, May 1, 2007; 176(1): 161 - 180.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Golovnin, A. Mazur, M. Kopantseva, M. Kurshakova, P. V. Gulak, B. Gilmore, W. G. F. Whitfield, P. Geyer, V. Pirrotta, and P. Georgiev
Integrity of the Mod(mdg4)-67.2 BTB Domain Is Critical to Insulator Function in Drosophila melanogaster
Mol. Cell. Biol., February 1, 2007; 27(3): 963 - 974.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. Gabler, M. Volkmar, S. Weinlich, A. Herbst, P. Dobberthien, S. Sklarss, L. Fanti, S. Pimpinelli, H. Kress, G. Reuter, et al.
Trans-splicing of the mod(mdg4) Complex Locus Is Conserved Between the Distantly Related Species Drosophila melanogaster and D. virilis
Genetics, February 1, 2005; 169(2): 723 - 736.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Melnick, G. Carlile, K. F. Ahmad, C.-L. Kiang, C. Corcoran, V. Bardwell, G. G. Prive, and J. D. Licht
Critical Residues within the BTB Domain of PLZF and Bcl-6 Modulate Interaction with Corepressors
Mol. Cell. Biol., March 15, 2002; 22(6): 1804 - 1818.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
K. Shim, K. J. Blake, J. Jack, and M. A. Krasnow
The Drosophila ribbon gene encodes a nuclear BTB domain protein that promotes epithelial migration and morphogenesis
Development, December 1, 2001; 128(23): 4923 - 4933.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
W. Wei and M. D. Brennan
The gypsy Insulator Can Act as a Promoter-Specific Transcriptional Stimulator
Mol. Cell. Biol., November 15, 2001; 21(22): 7714 - 7720.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Gause, P. Morcillo, and D. Dorsett
Insulation of Enhancer-Promoter Communication by a Gypsy Transposon Insert in the Drosophila cut Gene: Cooperation between Suppressor of Hairy-wing and Modifier of mdg4 Proteins
Mol. Cell. Biol., July 15, 2001; 21(14): 4807 - 4817.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Dorn, G. Reuter, and A. Loewendorf
Transgene analysis proves mRNA trans-splicing at the complex mod(mdg4) locus in Drosophila
PNAS, August 14, 2001; 98(17): 9724 - 9729.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.