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Nucleic Acids Research, 2000, Vol. 28, No. 21 4090-4096
© 2000 Oxford University Press

The yeast CDP1 gene encodes a triple-helical DNA-binding protein

Marco Musso, Giovanna Bianchi-Scarrà and Michael W. Van Dyke1,*

Dipartimento di Oncologia, Biologia e Genetica, Sezione di Biologia e Genetica, Università degli Studi di Genova, viale Benedetto XV, 6., 16132 Genova, Italy and 1Department of Molecular and Cellular Oncology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA

The formation of triple-helical DNA has been implicated in several cellular processes, including transcription, replication and recombination. While there is no direct evidence for triplexes in vivo, cellular proteins that specifically recognize triplex DNA have been described. Using a purine-motif triplex probe and southwestern library screening, we isolated five independent clones expressing the same C-terminal 210 amino acids of the Saccharomyces cerevisiae protein Cdp1p fused with ß-galactosidase. In electrophoretic mobility shift assays, recombinant Cdp1p{Delta}1-867 bound Pu-motif triplex DNAs with high affinity (Kd ~5 nM) and bound Py-motif triplex, duplex and single-stranded DNAs with far lower affinity (0.5–5.0 µM). Genetic analyses revealed that the CDP1 gene product was required for proper chromosome segregation. The possible involvement of triplex DNA in this process is discussed.

* To whom correspondence should be addressed. Tel: +1 713 792 8954; Fax: +1 713 794 0209; Email: mishko@odin.mdacc.tmc.edu


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