Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (754K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (41)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Hartung, F.
Right arrow Articles by Puchta, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hartung, F.
Right arrow Articles by Puchta, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2000, Vol. 28, No. 21 4275-4282
© 2000 Oxford University Press

Molecular characterisation of RecQ homologues in Arabidopsis thaliana

Frank Hartung*, Helena Plchová and Holger Puchta

Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstraße 3, D-06466 Gatersleben, Germany

Members of the RecQ family of DNA helicases are involved in processes linked to DNA replication, DNA recombination and gene silencing. RecQ homologues of various animals have been described recently. Here, for the first time for plants, we characterised cDNAs of all in all six different RecQ-like proteins that are expressed to different extents in Arabidopsis thaliana. Surprisingly, three of these proteins are small in size [AtRecQl1, AtRecQl2, AtRecQl3—606, 705 and 713 amino acids (aa), respectively], whereas the two bigger proteins result from a duplication event during plant evolution [AtRecQl4A and AtRecQl4B—1150 and 1182 aa, respectively]. Another homologue (AtRecQsim, 858 aa) most probably arose by insertion of an unrelated sequence within its helicase domain. The presence of these homologues demonstrates the conservation of RecQ family functions in higher eukaryotes. We also detected a small gene (AtWRNexo) encoding 285 aa which, being devoid of any RecQ-like helicase domain, reveals a striking homology to the exonuclease domain of human Werner protein, a prominent RecQ helicase of larger size. By means of the two-hybrid assay we were able to detect an interaction between AtWRNexo and AtRecQl2, indicating that activities that reside in a single protein chain in mammals might in plants be complemented in trans.

* To whom correspondence should be addressed. Tel: +49 39482 5248; Fax: +49 39482 5137; Email: hartung@ipk-gatersleben.de


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
D. Kobbe, S. Blanck, M. Focke, and H. Puchta
Biochemical Characterization of AtRECQ3 Reveals Significant Differences Relative to Other RecQ Helicases
Plant Physiology, November 1, 2009; 151(3): 1658 - 1666.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Lin, B. Hu, L. Chen, P. Sun, Y. Fan, P. Wu, and X. Chen
Computational Identification of Potential Molecular Interactions in Arabidopsis
Plant Physiology, September 1, 2009; 151(1): 34 - 46.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
I-P. Chen, A. Mannuss, N. Orel, F. Heitzeberg, and H. Puchta
A Homolog of ScRAD5 Is Involved in DNA Repair and Homologous Recombination in Arabidopsis
Plant Physiology, April 1, 2008; 146(4): 1786 - 1796.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Hartung, S. Suer, and H. Puchta
Two closely related RecQ helicases have antagonistic roles in homologous recombination and DNA repair in Arabidopsis thaliana
PNAS, November 20, 2007; 104(47): 18836 - 18841.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
F. Hartung, S. Suer, T. Bergmann, and H. Puchta
The role of AtMUS81 in DNA repair and its genetic interaction with the helicase AtRecQ4A
Nucleic Acids Res., September 11, 2006; 34(16): 4438 - 4448.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
B. Li, N. Conway, S. Navarro, L. Comai, and L. Comai
A conserved and species-specific functional interaction between the Werner syndrome-like exonuclease atWEX and the Ku heterodimer in Arabidopsis
Nucleic Acids Res., December 7, 2005; 33(21): 6861 - 6867.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Cui, D. Arosio, K. M. Doherty, R. M. Brosh Jr, A. Falaschi, and A. Vindigni
Analysis of the unwinding activity of the dimeric RECQ1 helicase in the presence of human replication protein A
Nucleic Acids Res., April 19, 2004; 32(7): 2158 - 2170.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Plchova, F. Hartung, and H. Puchta
Biochemical Characterization of an Exonuclease from Arabidopsis thaliana Reveals Similarities to the DNA Exonuclease of the Human Werner Syndrome Protein
J. Biol. Chem., November 7, 2003; 278(45): 44128 - 44138.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
N. Tuteja
Plant DNA helicases: the long unwinding road
J. Exp. Bot., October 1, 2003; 54(391): 2201 - 2214.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
T. Schwarzacher
Meiosis, recombination and chromosomes: a review of gene isolation and fluorescent in situ hybridization data in plants
J. Exp. Bot., January 1, 2003; 54(380): 11 - 23.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
F. Hartung, F. R. Blattner, and H. Puchta
Intron gain and loss in the evolution of the conserved eukaryotic recombination machinery
Nucleic Acids Res., December 1, 2002; 30(23): 5175 - 5181.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. S. Kamath-Loeb, L. A. Loeb, E. Johansson, P. M. J. Burgers, and M. Fry
Interactions between the Werner Syndrome Helicase and DNA Polymerase delta Specifically Facilitate Copying of Tetraplex and Hairpin Structures of the d(CGG)n Trinucleotide Repeat Sequence
J. Biol. Chem., May 4, 2001; 276(19): 16439 - 16446.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.