Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (202K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (27)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Petit, M.-A.
Right arrow Articles by Ehrlich, S. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Petit, M.-A.
Right arrow Articles by Ehrlich, S. D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2000, Vol. 28, No. 23 4642-4648
© 2000 Oxford University Press

The NAD-dependent ligase encoded by yerG is an essential gene of Bacillus subtilis

Marie-Agnès Petit* and S. Dusko Ehrlich

Laboratoire de Génétique Microbienne, INRA, 78352 Jouy en Josas, France

DNA ligases are grouped into two families, ATP-dependent and NAD-dependent, according to the cofactor required for their activity. A surprising capability of both kinds of ligases to complement for one another in vivo has been observed. Bacillus subtilis harbours one NAD-dependent ligase, YerG, and two ATP-dependent ligases, YkoU and YoqV, this last one being encoded by the 134 kb lysogenic bacteriophage SPß and consisting of a single adenylation domain typical of ATP-dependent ligases. Because the genetics of ligases in B.subtilis had not been studied previously, the genes encoding for one ligase of each kind, yerG and yoqV, were investigated. We found that the yerG gene was essential in B.subtilis. This suggests that none of the ATP-dependent ligases was able to complement the yerG defect. In addition, the ATP-dependent ligase encoded by yoqV, when cloned on a plasmid under appropriate expression signals, was unable to rescue a yerG mutant strain. The two B.subtilis ligase genes yerG and yoqV were also introduced in an Escherichia coli strain encoding a thermosensitive ligase (ligts), and whereas yoqV did not complement the ligts defects, yerG fully complemented the growth and UV sensitivity defects of the lig mutant. We propose to rename the yerG and yoqV genes of B.subtilis ligA and ligB respectively.

* To whom correspondence should be addressed. Tel: +33 1 34 65 25 12; Fax: +33 1 34 65 25 21; Email: petit{at}biotec.jouy.inra.fr


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
M. Korycka-Machala, E. Rychta, A. Brzostek, H. R. Sayer, A. Rumijowska-Galewicz, R. P. Bowater, and J. Dziadek
Evaluation of NAD+-Dependent DNA Ligase of Mycobacteria as a Potential Target for Antibiotics
Antimicrob. Agents Chemother., August 1, 2007; 51(8): 2888 - 2897.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Zhu and S. Shuman
Characterization of Agrobacterium tumefaciens DNA ligases C and D
Nucleic Acids Res., June 28, 2007; 35(11): 3631 - 3645.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. K. Srivastava, D. Dube, N. Tewari, N. Dwivedi, R. P. Tripathi, and R. Ramachandran
Mycobacterium tuberculosis NAD+-dependent DNA ligase is selectively inhibited by glycosylamines compared with human DNA ligase I
Nucleic Acids Res., December 15, 2005; 33(22): 7090 - 7101.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. K. Srivastava, R. P. Tripathi, and R. Ramachandran
NAD+-dependent DNA Ligase (Rv3014c) from Mycobacterium tuberculosis: CRYSTAL STRUCTURE OF THE ADENYLATION DOMAIN AND IDENTIFICATION OF NOVEL INHIBITORS
J. Biol. Chem., August 26, 2005; 280(34): 30273 - 30281.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Zhu and S. Shuman
Structure-guided Mutational Analysis of the Nucleotidyltransferase Domain of Escherichia coli NAD+-dependent DNA Ligase (LigA)
J. Biol. Chem., April 1, 2005; 280(13): 12137 - 12144.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
M. Lavesa-Curto, H. Sayer, D. Bullard, A. MacDonald, A. Wilkinson, A. Smith, L. Bowater, A. Hemmings, and R. P. Bowater
Characterization of a temperature-sensitive DNA ligase from Escherichia coli
Microbiology, December 1, 2004; 150(12): 4171 - 4180.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Gong, A. Martins, P. Bongiorno, M. Glickman, and S. Shuman
Biochemical and Genetic Analysis of the Four DNA Ligases of Mycobacteria
J. Biol. Chem., May 14, 2004; 279(20): 20594 - 20606.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Brotz-Oesterhelt, I. Knezevic, S. Bartel, T. Lampe, U. Warnecke-Eberz, K. Ziegelbauer, D. Habich, and H. Labischinski
Specific and Potent Inhibition of NAD+-dependent DNA Ligase by Pyridochromanones
J. Biol. Chem., October 10, 2003; 278(41): 39435 - 39442.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
G. R. Weller, B. Kysela, R. Roy, L. M. Tonkin, E. Scanlan, M. Della, S. K. Devine, J. P. Day, A. Wilkinson, F. d'A. di Fagagna, et al.
Identification of a DNA Nonhomologous End-Joining Complex in Bacteria
Science, September 6, 2002; 297(5587): 1686 - 1689.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Sriskanda and S. Shuman
Conserved Residues in Domain Ia Are Required for the Reaction of Escherichia coli DNA Ligase with NAD+
J. Biol. Chem., March 15, 2002; 277(12): 9695 - 9700.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. Sriskanda and S. Shuman
A second NAD+-dependent DNA ligase (LigB) in Escherichia coli
Nucleic Acids Res., December 15, 2001; 29(24): 4930 - 4934.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Sriskanda, R. W. Moyer, and S. Shuman
NAD+-dependent DNA Ligase Encoded by a Eukaryotic Virus
J. Biol. Chem., September 21, 2001; 276(39): 36100 - 36109.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. M. Olland, K. W. Underwood, R. M. Czerwinski, M.-C. Lo, A. Aulabaugh, J. Bard, M. L. Stahl, W. S. Somers, F. X. Sullivan, and R. Chopra
Identification, Characterization, and Crystal Structure of Bacillus subtilis Nicotinic Acid Mononucleotide Adenylyltransferase
J. Biol. Chem., January 25, 2002; 277(5): 3698 - 3707.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.