Nucleic Acids Research, 2000, Vol. 28, No. 24 4846-4855
© 2000 Oxford University Press
Characterization of the B lymphocyte-induced maturation protein-1 (Blimp-1) gene, mRNA isoforms and basal promoter
1Department of Microbiology and 2Integrated Program in Biophysical, Cellular and Molecular Studies, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
Blimp-1 is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Here we report the organization of the mouse Blimp-1 gene, an analysis of Blimp-1 homologs in different species, the characterization of Blimp-1 mRNA isoforms and initial studies on the transcription of Blimp-1. The murine Blimp-1 gene covers ~23 kb and contains eight exons. There are Blimp-1 homologs in species evolutionarily distant from mouse (Caenorhabditis elegans and Drosophila melanogaster) but no homolog was found in the unicellular yeast Saccharomyces cerevisiae. The three major Blimp-1 mRNA isoforms result from the use of different polyadenylation sites and do not encode different proteins. Run-on transcription analyses were used to show that the developmentally regulated expression of Blimp-1 mRNA in B cells is determined by transcription initiation. Multiple Blimp-1 transcription initiates sites were mapped near an initiator element and a region conferring basal promoter activity has been identified.
* To whom correspondence should be addressed at: 701 West 168th Street, Columbia University, HHSC 1202, New York, NY 10032, USA. Tel: +1 212 305 3504; Fax: +1 212 305 1468; Email: klcl{at}columbia.edu
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