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Nucleic Acids Research, 2000, Vol. 28, No. 24 4865-4872
© 2000 Oxford University Press

A novel four zinc-finger protein targeted against p190BcrAbl fusion oncogene cDNA: utilisation of zinc-finger recognition codes

Andrew R. McNamara and Kevin G. Ford*

Department of Molecular Medicine, The Rayne Institute, Guy’s, King’s and St Thomas’ Schools of Medicine and Dentistry, 123 Coldharbour Lane, Camberwell, London SE5 9NU, UK

A three zinc-finger protein that binds specifically to the cDNA representing the unique fusion gene BcrAbl, associated with acute lymphoblastic leukaemia, has previously been characterised. At this breakpoint, a sequence homology of 8/9 bp exists between the BcrAbl (fusion) and c-Abl (parental) target sequences. We show that the three zinc-finger protein discriminates poorly between the fusion (BcrAbl) and parental (Abl) sequence (Kds of 42.8 and 65.1 nM, respectively). In order to improve the discriminatory properties of this protein, and to demonstrate the utility of current zinc-finger databases, we have added a fourth zinc-finger to the original three zinc-finger protein. This fourth finger recognises a 3 bp subsite derived from the Bcr portion of the breakpoint and is not present in c-Abl. This novel four finger protein, which now recognises a 12 bp sequence, demonstrates improved specific binding to BcrAbl (Kd = 17 nM). More significantly we have shown that there is now enhanced discrimination between BcrAbl and Abl sequences by the four finger protein than the original three finger protein.

* To whom correspondence should be addressed. Tel: +44 207 848 5907; Fax: +44 207 733 3877; Email: kevin.ford{at}kcl.ac.uk


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A. R. McNamara, P. J. Hurd, A. E. F. Smith, and K. G. Ford
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