Nucleic Acids Research, 2000, Vol. 28, No. 4 960-967
© 2000 Oxford University Press
Cloning and DNA-binding properties of a tobacco Ethylene-Insensitive3 (EIN3) homolog
National Institute of Agrobiological Resources, Tsukuba, Ibaraki 305-8602, Japan
Ethylene-Insensitive3 (EIN3) is a transcription factor that works in the ethylene signaling pathway in Arabidopsis. We isolated a tobacco cDNA encoding an EIN3 homolog as a sequence-specific DNA-binding protein. The encoded protein TEIL (tobacco EIN3-like) shares 60% identity in amino acid sequence with EIN3. The DNA-binding domain was localized in the N-terminal half, which shows 92% identity in amino acid sequence with the corresponding region of EIN3, suggesting a conserved function in DNA-binding specificity. TEIL was indeed functionally similar to EIN3 because, like EIN3-overexpressing plants, transgenic Arabidopsis seedlings overexpressing TEIL cDNA exhibited constitutive triple response phenotypes. Random binding site selection analysis revealed that the consensus binding sequence for TEIL is AYGWAYCT, where Y and W represent A or C and A or T, respectively. A reporter plasmid containing the TEIL binding sites showed a 7- to 10-fold higher activation relative to that containing a mutated TEIL-binding sequence in tobacco protoplasts. A further 2- to 3-fold increase in activation was observed when a plasmid for TEIL overproduction was co-transfected, indicating that TEIL is a transcriptional activator. Moreover, nuclear extracts from ethylene-treated leaves showed an increase in DNA-binding activity specific to the TEIL-binding sequence, despite the level of the transcripts being unchanged. These observations suggest that TEIL functions as a transcription activator with a relatively redundant DNA-binding specificity, and its function may be regulated at least in part by modulation of the DNA-binding activity through ethylene signaling.
* To whom correspondence should be addressed. Tel: +81 298 38 7440; Fax: +81 298 38 7469; Email: yohashi@abr.affrc.go.jp
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Wang, B. Zhang, X. Li, C. Xu, X. Yin, L. Shan, I. Ferguson, and K. Chen Ethylene signal transduction elements involved in chilling injury in non-climacteric loquat fruit J. Exp. Bot., October 30, 2009; (2009) erp302v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Chen, L. Xue, S. Chintamanani, H. Germain, H. Lin, H. Cui, R. Cai, J. Zuo, X. Tang, X. Li, et al. ETHYLENE INSENSITIVE3 and ETHYLENE INSENSITIVE3-LIKE1 Repress SALICYLIC ACID INDUCTION DEFICIENT2 Expression to Negatively Regulate Plant Innate Immunity in Arabidopsis PLANT CELL, August 1, 2009; 21(8): 2527 - 2540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hibi, S. Kosugi, T. Iwai, M. Kawata, S. Seo, I. Mitsuhara, and Y. Ohashi Involvement of EIN3 homologues in basic PR gene expression and flower development in tobacco plants J. Exp. Bot., October 26, 2007; (2007) erm216v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Maruyama-Nakashita, Y. Nakamura, T. Tohge, K. Saito, and H. Takahashi Arabidopsis SLIM1 Is a Central Transcriptional Regulator of Plant Sulfur Response and Metabolism PLANT CELL, November 1, 2006; 18(11): 3235 - 3251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ma, H. Tan, X. Liu, J. Xue, Y. Li, and J. Gao Transcriptional regulation of ethylene receptor and CTR genes involved in ethylene-induced flower opening in cut rose (Rosa hybrida) cv. Samantha J. Exp. Bot., August 1, 2006; 57(11): 2763 - 2773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Iordachescu and S. Verlinden Transcriptional regulation of three EIN3-like genes of carnation (Dianthus caryophyllus L. cv. Improved White Sim) during flower development and upon wounding, pollination, and ethylene exposure J. Exp. Bot., August 1, 2005; 56(418): 2011 - 2018. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. O. Steffens, C. Galuschka, M. Schindler, L. Bulow, and R. Hehl AthaMap web tools for database-assisted identification of combinatorial cis-regulatory elements and the display of highly conserved transcription factor binding sites in Arabidopsis thaliana Nucleic Acids Res., July 1, 2005; 33(suppl_2): W397 - W402. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ogawa, L. Pan, M. Kawai-Yamada, L.-H. Yu, S. Yamamura, T. Koyama, S. Kitajima, M. Ohme-Takagi, F. Sato, and H. Uchimiya Functional Analysis of Arabidopsis Ethylene-Responsive Element Binding Protein Conferring Resistance to Bax and Abiotic Stress-Induced Plant Cell Death Plant Physiology, July 1, 2005; 138(3): 1436 - 1445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. N. Stepanova and J. M. Alonso Ethylene Signaling Pathway Sci. Signal., March 22, 2005; 2005(276): cm3 - cm3. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Chen, L. Alexander, and D. Grierson Constitutive expression of EIL-like transcription factor partially restores ripening in the ethylene-insensitive Nr tomato mutant J. Exp. Bot., July 1, 2004; 55(402): 1491 - 1497. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. O. Steffens, C. Galuschka, M. Schindler, L. Bulow, and R. Hehl AthaMap: an online resource for in silico transcription factor binding sites in the Arabidopsis thaliana genome Nucleic Acids Res., January 1, 2004; 32(90001): D368 - 372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Yokotani, S. Tamura, R. Nakano, A. Inaba, and Y. Kubo Characterization of a novel tomato EIN3-like gene (LeEIL4) J. Exp. Bot., December 1, 2003; 54(393): 2775 - 2776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Rieu, C. Mariani, and K. Weterings Expression analysis of five tobacco EIN3 family members in relation to tissue-specific ethylene responses J. Exp. Bot., October 1, 2003; 54(391): 2239 - 2244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kosugi and Y. Ohashi Constitutive E2F Expression in Tobacco Plants Exhibits Altered Cell Cycle Control and Morphological Change in a Cell Type-Specific Manner Plant Physiology, August 1, 2003; 132(4): 2012 - 2022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Lee and W. T. Kim Molecular and Biochemical Characterization of VR-EILs Encoding Mung Bean ETHYLENE INSENSITIVE3-LIKE Proteins Plant Physiology, July 1, 2003; 132(3): 1475 - 1488. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Alonso, A. N. Stepanova, R. Solano, E. Wisman, S. Ferrari, F. M. Ausubel, and J. R. Ecker Five components of the ethylene-response pathway identified in a screen for weak ethylene-insensitive mutants in Arabidopsis PNAS, March 4, 2003; 100(5): 2992 - 2997. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kosugi and Y. Ohashi E2Ls, E2F-like Repressors of Arabidopsis That Bind to E2F Sites in a Monomeric Form J. Biol. Chem., May 3, 2002; 277(19): 16553 - 16558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L.-C. Wang, H. Li, and J. R. Ecker Ethylene Biosynthesis and Signaling Networks PLANT CELL, May 1, 2002; 14(90001): S131 - 151. [Full Text] [PDF]
|
|
|
|
|
|
S. H. Yang, T. Berberich, H. Sano, and T. Kusano Specific Association of Transcripts of tbzF and tbz17, Tobacco Genes Encoding Basic Region Leucine Zipper-Type Transcriptional Activators, with Guard Cells of Senescing Leaves and/or Flowers Plant Physiology, September 1, 2001; 127(1): 23 - 32. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Waki, K. Shibuya, T. Yoshioka, T. Hashiba, and S. Satoh Cloning of a cDNA encoding EIN3-like protein (DC-EIL1) and decrease in its mRNA level during senescence in carnation flower tissues J. Exp. Bot., February 1, 2001; 52(355): 377 - 379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ohme-Takagi, K. Suzuki, and H. Shinshi Regulation of Ethylene-Induced Transcription of Defense Genes Plant Cell Physiol., November 1, 2000; 41(11): 1187 - 1192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kosugi and Y. Ohashi Interaction of the Arabidopsis E2F and DP Proteins Confers Their Concomitant Nuclear Translocation and Transactivation Plant Physiology, March 1, 2002; 128(3): 833 - 843. [Abstract] [Full Text] [PDF]
|
|