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Nucleic Acids Research, 2000, Vol. 28, No. 4 974-981
© 2000 Oxford University Press

Stp1p, Stp2p and Abf1p are involved in regulation of expression of the amino acid transporter gene BAP3 of Saccharomyces cerevisiae

Marco de Boer, Peter S. Nielsen1, Jan-Paul Bebelman, Harm van Heerikhuizen, Helge A. Andersen1 and Rudi J. Planta*

Department of Biochemistry and Molecular Biology, Faculty of Sciences, BioCentrum Amsterdam, Vrije Universiteit, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands and 1Carlsberg Laboratory, Department of Yeast Genetics, Gamle Carlsberg Vej 10, DK-2500, Copenhagen Valby, Denmark

Expression of the BAP3 gene of Saccharomyces cerevisiae, encoding a branched chain amino acid permease, is induced in response to the availability of several naturally occurring amino acids in the medium. This induction is mediated via an upstream activating sequence (called UASaa) in the BAP3 promoter, and dependent on Stp1p, a nuclear protein with zinc finger domains, suggesting that Stp1p is a transcription factor involved in BAP3 expression. In this paper, we show that Stp2p, a protein with considerable similarity to Stp1p, is also involved in the induction of BAP3 expression. To gain more insight into the roles of STP1 and STP2, we have overexpressed both Stp1p and Stp2p in yeast cells. Gel shift assays with the UASaa as a probe show that the UASaa can form two major complexes. One complex is dependent on Stp2p overexpression and the other is formed independently of STP1 or STP2, suggesting that the UASaa is also bound by another factor. Here we show that the other factor is Abf1p, which binds specifically to the UASaa of BAP3.

* To whom correspondence should be addressed. Tel. +31 20 444 7555; Fax +31 20 444 7553; Email: brink@chem.vu.nl


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