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Nucleic Acids Research, 2000, Vol. 28, No. 4 E12-e12
© 2000 Oxford University Press

A simplified method of generating transgenic Xenopus

Duncan B. Sparrow*, Branko Latinkic and Tim J. Mohun

Division of Developmental Biology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK

Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos.

* To whom correspondence should be addressed. Tel: +44 181 913 8621; Fax: +44 181 906 4477; Email: dsparro@nimr.mrc.ac.uk


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