Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (263K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (34)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Rang, A.
Right arrow Articles by Will, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rang, A.
Right arrow Articles by Will, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2000, Vol. 28, No. 5 1120-1125
© 2000 Oxford University Press

The tetracycline-responsive promoter contains functional interferon-inducible response elements

Andreas Rang and Hans Will*

Heinrich-Pette-Institut, Martinistraße 52, D-20251 Hamburg, Germany

Tetracycline (tet)-responsive expression vectors allow controlled inducible expression of proteins in mammalian cells. This system is widely used for experimental research both in vivo and in vitro. In our attempts to use this system to study the antiviral effect of IFN{alpha} on hepatitis B virus, we discovered an unexpected feature of the tet-responsive promoter (tet promoter) of the currently available expression vectors. IFN{alpha} was found to stimulate tet promoter activity after transient transfection in a dose- and cell type-dependent manner. By sequence inspection, an IFN{alpha}-stimulated response element (ISRE)-like sequence was identified in the linker regions located between the heptameric tet operator sequences. Gel shift assays revealed binding of IFN-stimulated gene factors to these sequences, indicating that they mediate the IFN{alpha}-mediated promoter stimulation. These data demonstrate an unexpected feature of the tet-responsive expression system which needs to be taken into acount when using this system for analysis of cytokine functions in vitro and in vivo. The data also imply that the tet promoter-based expression system can be rendered non-responsive to IFN{alpha} by mutagenesis of the ISREs and this may be essential when considering gene therapy in vivo.

* To whom correspondence should be addressed. Tel: +49 40 48051 221; Fax: +49 40 48051 222; Email: will@hpi.uni-hamburg.de


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GeneticsHome page
M. Bao-Cutrona and P. Moral
Unexpected Expression Pattern of Tetracycline-Regulated Transgenes in Mice
Genetics, April 1, 2009; 181(4): 1687 - 1691.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
W. Weber, W. Bacchus, M. Daoud-El Baba, and M. Fussenegger
Vitamin H-regulated transgene expression in mammalian cells
Nucleic Acids Res., September 27, 2007; 35(17): e116 - e116.
[Abstract] [Full Text] [PDF]

Home page
 Brief Funct Genomic ProteomicHome page
M. J. Osborn and J. R. Miller
Rescuing yeast mutants with human genes
Brief Funct Genomic Proteomic, August 13, 2007; (2007) elm017v1.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. S. Kandel, T. Lu, Y. Wan, M. K. Agarwal, M. W. Jackson, and G. R. Stark
Mutagenesis by reversible promoter insertion to study the activation of NF-{kappa}B
PNAS, May 3, 2005; 102(18): 6425 - 6430.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Ehlers, S. Horke, K. Reumann, A. Rang, F. Grosse, H. Will, and T. Heise
Functional Characterization of the Interaction between Human La and Hepatitis B Virus RNA
J. Biol. Chem., October 15, 2004; 279(42): 43437 - 43447.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
G. Marzio, M. Vink, K. Verhoef, A. de Ronde, and B. Berkhout
Efficient Human Immunodeficiency Virus Replication Requires a Fine-Tuned Level of Transcription
J. Virol., February 22, 2002; 76(6): 3084 - 3088.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Rang, T. Heise, and H. Will
Lack of a Role of the Interferon-stimulated Response Element-like Region in Interferon alpha -induced Suppression of Hepatitis B Virus in Vitro
J. Biol. Chem., January 26, 2001; 276(5): 3531 - 3535.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.