Nucleic Acids Research, 2000, Vol. 28, No. 6 1299-1306
© 2000 Oxford University Press
Intronic GIY-YIG endonuclease gene in the mitochondrial genome of Podospora curvicolla: evidence for mobility
Centre de Génétique Moléculaire, CNRS, Avenue de la Terrasse, 91198 Gif Sur Yvette Cedex, France and 1Université de Versailles/Saint-Quentin-en-Yvelines UPRESA 8087 Laboratoire Génome, Cellule et Informatique, Bâtiment Fermat, 45 avenue des Etats-Unis, 78035 Versailles Cedex, France
Endonuclease genes encoded in invasive introns are themselves supposed to be mobile elements which, during evolution, have colonized pre-existing introns converting them into invasive elements. This hypothesis is supported by numerous data concerning the LAGLI-DADG subclass of intronic endonucleases. Less is known about the GIY-YIG ORFs which constitute another family of endonucleases. In this paper we describe the presence of one optional GIY-YIG ORF in the second intron of the mitochondrial cytochrome b gene in the fungus Podospora curvicolla. We show that this GIY-YIG ORF is efficiently transferred from an ORF-containing intron to an ORF-less allele. We also show that the products of both the GIY-YIG ORF and the non-canonical LAGLI-DADG-GIY-YIG ORF, which is generated by its integration, have endonuclease activities which recognize and cut the insertion site of the optional sequence. This constitutes the first direct evidence for potential mobility of an intronic GIY-YIG endonuclease. We discuss the role that such a mobile sequence could have played during evolution.
* To whom correspondence should be addressed. Tel: +33 01 69 82 3145; Fax: +33 01 69 82 3150; Email: koll@cgm.cnrs-gif.fr
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