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Nucleic Acids Research, 2000, Vol. 28, No. 6 1439-1446
© 2000 Oxford University Press

The initiator element of the Drosophila ß2 tubulin gene core promoter contributes to gene expression in vivo but is not required for male germ-cell specific expression

Ansgar Santel*, Jörg Kaufmann1, Ruth Hyland and Renate Renkawitz-Pohl

Zoologie-Entwicklungsbiologie am Fachbereich Biologie, Philipps-Universität Marburg, Karl-von-Frisch-Straße, D-35032 Marburg, Germany and 1Chiron Corporation, Chiron Technologies, 4560 Horton Street, Emeryville, CA 94608, USA

The tissue-specific expression of the Drosophila ß2 tubulin gene (B2t) is accomplished by the action of a 14-bp activator element (ß2UE1) in combination with certain regulatory elements of the TATA-less, Inr-containing B2t core promoter. We performed an in vivo analysis of the Inr element function in the B2t core promoter using a transgenic approach. Our experiments demonstrate that the Inr element acts as a functional cis-regulatory element in vivo and quantitatively regulates tissue-specific reporter expression in transgenic animals. However, our mutational analysis of the Inr element demonstrates no essential role of the Inr in mediating tissue specificity of the B2t promoter. In addition, a downstream element seems to affect promoter activity in combination with the Inr. In summary, our data show for the first time the functionality of the Inr element in an in vivo background situation in Drosophila.

* To whom correspondence should be addressed at present address: Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA. Tel: +1 650 725 7625; Fax: +1 650 725 7739; Email: santel@cmgm.stanford.edu


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