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Nucleic Acids Research, 2000, Vol. 28, No. 8 E29-00
© 2000 Oxford University Press

Advanced method for oligonucleotide deprotection

Sergey A. Surzhikov, Edward N. Timofeev, Boris K. Chernov, Julia B. Golova and Andrei D. Mirzabekov*

Joint Human Genome Program, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 117984 Moscow, Russia and Argonne National Laboratory, Argonne, IL 60439, USA

A new procedure for rapid deprotection of synthetic oligodeoxynucleotides has been developed. While all known deprotection methods require purification to remove the residual protective groups (e.g. benzamide) and insoluble silicates, the new procedure based on the use of an ammonia-free reagent mixture allows one to avoid the additional purification steps. The method can be applied to deprotect the oligodeoxynucleotides synthesized by using the standard protected nucleoside phosphoramidites dGiBu, dCBz and dABz.

* To whom correspondence should be addressed at: Biochip Technology Center, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439-4833, USA. Tel: +1 630 252 3161; Fax: +1 630 252 9155; Email: amir@everest.bim.anl.gov


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