Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (94K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Capaldi, D. C.
Right arrow Articles by Ravikumar, V. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Capaldi, D. C.
Right arrow Articles by Ravikumar, V. T.
Related Collections
Right arrow Nucleic acid modification
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2000, Vol. 28, No. 9 E40-e40
© 2000 Oxford University Press

Highly efficient solid phase synthesis of oligonucleotide analogs containing phosphorodithioate linkages

Daniel C. Capaldi, Douglas L. Cole and Vasulinga T. Ravikumar*

Isis Pharmaceuticals, 2292 Faraday Avenue, Carlsbad, CA 92008, USA

A triester method for the synthesis of deoxynucleoside phosphorodithioate dimers is described. The phosphorodithioate linkage is introduced using a new dithiophosphorylating reagent DPSE-SP(S)Cl2 where DPSE = 2-diphenylmethylsilylethyl. This group is removed quickly using tetra-butylammonium fluoride leading to the quantitative formation of phosphoro­dithioate diesters uncontaminated with the corresponding phosphorothioates. The utility of this group is demonstrated by the synthesis of a penta­decathymidylic acid, [T(PS2)T(PO2)]7T, which contains alternating phosphorodithioate/phosphate diester internucleotide linkages.

* To whom correspondence should be addressed. Tel: +1 760 603 2412; Fax: +1 760 929 0528; Email: vravikumar@isisph.com


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.