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Nucleic Acids Research, 2001, Vol. 29, No. 1 178-180
© 2001 Oxford University Press

RISSC: a novel database for ribosomal 16S–23S RNA genes spacer regions

Jesús García-Martínez, Ignacio Bescós, Jesús Javier Rodríguez-Sala1 and Francisco Rodríguez-Valera*

División de Microbiología, Campus de San Juan and 1Centro de Cálculo, Edificio de La Gal·lia, Universidad Miguel Hernández, Elche, Alicante, Spain

A novel database, under the acronym RISSC (Ribosomal Intergenic Spacer Sequence Collection), has been created. It compiles more than 1600 entries of edited DNA sequence data from the 16S–23S ribosomal spacers present in most prokaryotes and organelles (e.g. mitochondria and chloroplasts) and is accessible through the Internet (http://ulises.umh.es/RISSC), where systematic searches for specific words can be conducted, as well as BLAST-type sequence searches. Additionally, a characteristic feature of this region, the presence/absence and nature of tRNA genes within the spacer, is included in all the entries, even when not previously indicated in the original database. All these combined features could provide a useful documen­tation tool for studies on evolution, identification, typing and strain characterization, among others.

* To whom correspondence should be addressed at: División de Microbiología, Universidad Miguel Hernández, Campus de San Juan, Carretera de Valencia Km 87, Apartado 18, 03550 San Juan, Alicante, Spain. Tel: +34 965 919 451; Fax: +34 965 919 457; Email: frvalera{at}umh.es


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