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Nucleic Acids Research, 2001, Vol. 29, No. 10 2020-2025
© 2001 Oxford University Press

The Saccharomyces cerevisiae RAD9 cell cycle checkpoint gene is required for optimal repair of UV-induced pyrimidine dimers in both G1 and G2/M phases of the cell cycle

Nisreen M. Al-Moghrabi, Ibtehaj S. Al-Sharif and Abdelilah Aboussekhra*

King Faisal Specialist Hospital and Research Center, Department of Biological and Medical Research, MBC-03, PO Box 3354, Riyadh 11211, Kingdom of Saudi Arabia

Cells respond to DNA damage by activating both cellular growth arrest and DNA repair processes. In Saccharomyces cerevesiae the RAD9 gene controls DNA damage-mediated cell cycle arrest that is known to allow efficient repair. To ascertain whether RAD9 plays a role in DNA repair per se, the removal of UV-induced photolesions was assessed in synchronized isogenic normal and rad9{Delta} cells using the high resolution primer extension technique. The results show that RAD9 is indeed involved in the removal of photolesions from both the transcribed and the non-transcribed strands of the reporter GAL10 gene, in G1- as well as G2/M-arrested cells. Interestingly, these data also reveal that in both normal and rad9 mutant, the repair strand bias towards the transcribed stand is more pronounced in G2/M- than in G1-arrested cells. These data indicate that RAD9 coordinate the cellular response to DNA damage by activating both cell cycle checkpoint and excision repair.

* To whom correspondence should be addressed. Tel: +966 1 464 7272; Fax: +966 1 442 7858; Email: aboussekhra{at}kfshrc.edu.sa


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