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Nucleic Acids Research, 2001, Vol. 29, No. 10 e50
© 2001 Oxford University Press

The rational design of a ‘type 88’ genetically stable peptide display vector in the filamentous bacteriophage fd

David Enshell-Seijffers, Larisa Smelyanski and Jonathan M. Gershoni*

Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel

Filamentous bacteriophages are particularly efficient for the expression and display of combinatorial random peptides. Two phage proteins are often employed for peptide display: the infectivity protein, PIII, and the major coat protein, PVIII. The use of PVIII typically requires the expression of two pVIII genes: the wild-type and the recombinant pVIII gene, to generate mosaic phages. ‘Type 88’ vectors contain two pVIII genes in one phage genome. In this study a novel ‘type 88’ expression vector has been rationally designed and constructed. Two factors were taken into account: the insertion site and the genetic stability of the second pVIII gene. It was found that selective deletion of recombinant genes was encountered when inserts were cloned into either of the two non-coding regions of the phage genome. The deletions were independent of recA yet required a functional F-episome. Transcription was also found to be a positive factor for deletion. Taking the above into account led to the generation of a novel vector, designated fth1, which can be used to express recombinant peptides as pVIII chimeric proteins in mosaic bacteriophages. The fth1 vector is not only genetically stable but also of high copy number and produces high titers of recombinant phages.

* To whom correspondnce should be addressed. Tel: +972 3 640 8981; Fax: +972 3 642 2046; Email: gershoni{at}post.tau.ac.il


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