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Nucleic Acids Research, 2001, Vol. 29, No. 15 3212-3218
© 2001 Oxford University Press

Ribonucleotide reductase genes of Bacillus prophages: a refuge to introns and intein coding sequences

Vladimir Lazarevic*

Institut de Génétique et de Biologie Microbiennes, Rue César-Roux 19, CH-1005 Lausanne, Switzerland

The ribonucleotide reductase gene tandem bnrdE/bnrdF in SPß-related prophages of different Bacillus spp. isolates presents different configurations of intervening sequences, comprising one to three of six non-homologous splicing elements. Insertion sites of group I introns and intein DNA are clustered in three relatively short segments encoding functionally important domains of the ribonucleotide reductase. Comparison of the bnrdE homologs reveals mutual exclusion of a group I intron and an intein coding sequence flanking the codon that specifies a conserved cysteine. In vivo splicing was demonstrated for all introns. However, for two of them a part of the mRNA precursor molecules remains unspliced. Intergenic bnrdE–bnrdF regions are unexpectedly long, comprising between 238 and 541 nt. The longest encodes a putative polypeptide related to HNH homing endonucleases.

* Tel: +41 21 320 60 75; Fax: +41 21 320 60 78; Email: vladimir.lazarevic{at}igbm.unil.ch


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