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Nucleic Acids Research, 2001, Vol. 29, No. 2 380-386
© 2001 Oxford University Press

Conserved regulatory elements of the promoter sequence of the gene rpoH of enteric bacteria

Jesús Ramírez-Santos, Julio Collado-Vides1, Martin García-Varela2 and M. Carmen Gómez-Eichelmann*

Departamento de Biología Molecular, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, PO Box 70-228, 04510 México, D. F., México, 1Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, PO Box 565-A, 62100 Cuernavaca Morelos, México and 2Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, PO Box 70-228, 04510 México, D. F., México

The rpoH regulatory region of different members of the enteric bacteria family was sequenced or downloaded from GenBank and compared. In addition, the transcriptional start sites of rpoH of Yersinia frederiksenii and Proteus mirabilis, two distant members of this family, were determined. Sequences similar to the {sigma}70 promoters P1, P4 and P5, to the {sigma}E promoter P3 and to boxes DnaA1, DnaA2, cAMP receptor protein (CRP) boxes CRP1, CRP2 and box CytR present in Escherichia coli K12, were identified in sequences of closely related bacteria such as: E.coli, Shigella flexneri, Salmonella enterica serovar Typhimurium, Citrobacter freundii, Enterobacter cloacae and Klebsiella pneumoniae. In more distant bacteria, Y.frederiksenii and P.mirabilis, the rpoH regulatory region has a distal P1-like {sigma}70 promoter and two proximal promoters: a heat-induced {sigma}E-like promoter and a {sigma}70 promoter. Sequences similar to the regulatory boxes were not identified in these bacteria. This study suggests that the general pattern of transcription of the rpoH gene in enteric bacteria includes a distal {sigma}70 promoter, >200 nt upstream of the initiation codon, and two proximal promoters: a heat-induced {sigma}E-like promoter and a {sigma}70 promoter. A second proximal {sigma}70 promoter under catabolite-regulation is probably present only in bacteria closely related to E.coli.

* To whom correspondence should be addressed. Tel: + 52 5 622 38 52; Fax: +52 5 622 38 91; Email: cargom{at}servidor.unam.mx


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