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Nucleic Acids Research, 2001, Vol. 29, No. 2 e2
© 2001 Oxford University Press

Chemical nanoprinting: a novel method for fabricating DNA microchips

Anil Kumar and Zicai Liang*

Genomics Technology Unit, Center for Genomics Research, Karolinska Institutet, 17177 Stockholm, Sweden

We have developed a novel cost-effective procedure, namely ‘chemical nanoprinting’, for oligonucleotide or cDNA chips manufacture. In this thermo-controlled process, the oligonucleotides, covalently attached to a highly loaded ‘master-chip’ through disulfide bonds, are chemically transferred to the acrylamide layer mounted on a ‘print-chip’. It is demonstrated here that multiple identical print-chips can be produced from a single master-chip. This duplication process is a few hundreds of times faster than any existing methods and the speed of process and cost incurred are independent of the scale of the DNA chips.

* To whom correspondence should be addressed. Tel: +46 8 7286371; Fax: +46 8 331547; Email: zicai.liang{at}cgr.ki.se


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