Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (487K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (29)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Hirschler-Laszkiewicz, I.
Right arrow Articles by Rothblum, L. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hirschler-Laszkiewicz, I.
Right arrow Articles by Rothblum, L. I.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2001, Vol. 29, No. 20 4114-4124
© 2001 Oxford University Press

The role of acetylation in rDNA transcription

Iwona Hirschler-Laszkiewicz, Alice Cavanaugh, Qiyue Hu, Jason Catania1, Maria Laura Avantaggiati1 and Lawrence I. Rothblum*

The Henry Hood Research Program, Sigfried and Janet Weis Center for Research, The Geisinger Clinic, 100 North Academy Avenue, Danville, PA 17822-2618, USA and 1Department of Pharmacology and Therapeutics, Roswell Park Cancer Institute, Buffalo, NY 14263, USA

Treatment of NIH 3T3 cells with trichostatin A (TSA), an inhibitor of histone deacetylase (HDAC), resulted in a dose-dependent increase in transcription from a rDNA reporter and from endogenous rRNA genes. Chromatin immunoprecipitation using anti-acetyl-histone H4 antibodies demonstrated a direct effect of TSA on the acetylation state of the ribosomal chromatin. TSA did not reverse inhibition of transcription from the rDNA reporter by retinoblastoma (Rb) protein, suggesting that the main mechanism by which Rb blocks rDNA transcription may not involve recruitment of deacetylases to rDNA chromatin. Overexpression of histone transacetylases p300, CBP and PCAF stimulated transcription in transfected NIH 3T3 cells. Recombinant p300, but not PCAF, stimulated rDNA transcription in vitro in the absence of nucleosomes, suggesting that the stimulation of rDNA transcription by TSA might have a chromatin-independent component. We found that the rDNA transcription factor UBF was acetylated in vivo. Finally, we also demonstrated the nucleolar localization of CBP. Our results suggest that the organization of ribosomal chromatin of higher eukaryotes is not static and that acetylation may be involved in affecting these dynamic changes directly through histone acetylation and/or through acetylation of UBF or one of the other components of rDNA transcription.

* To whom correspondence should be addressed. Tel: +1 570 271 6662; Fax: +1 570 271 6701; Email: lrothblum{at}geisinger.edu


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 JCBHome page
E. Sanij, G. Poortinga, K. Sharkey, S. Hung, T. P. Holloway, J. Quin, E. Robb, L. H. Wong, W. G. Thomas, V. Stefanovsky, et al.
UBF levels determine the number of active ribosomal RNA genes in mammals
J. Cell Biol., December 29, 2008; 183(7): 1259 - 1274.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. Mertens and R. G. Roeder
Different Functional Modes of p300 in Activation of RNA Polymerase III Transcription from Chromatin Templates
Mol. Cell. Biol., September 15, 2008; 28(18): 5764 - 5776.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. E. Brown and M. Szyf
Epigenetic Programming of the rRNA Promoter by MBD3
Mol. Cell. Biol., July 1, 2007; 27(13): 4938 - 4952.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C.-Y. Lin, S. Navarro, S. Reddy, and L. Comai
CK2-mediated stimulation of Pol I transcription by stabilization of UBF-SL1 interaction
Nucleic Acids Res., October 18, 2006; 34(17): 4752 - 4766.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Majumder, K. Ghoshal, J. Datta, D. S. Smith, S. Bai, and S. T. Jacob
Role of DNA Methyltransferases in Regulation of Human Ribosomal RNA Gene Transcription
J. Biol. Chem., August 4, 2006; 281(31): 22062 - 22072.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Meraner, M. Lechner, A. Loidl, M. Goralik-Schramel, R. Voit, I. Grummt, and P. Loidl
Acetylation of UBF changes during the cell cycle and regulates the interaction of UBF with RNA polymerase I.
Nucleic Acids Res., January 1, 2006; 34(6): 1798 - 1806.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Shimono, Y. Shimono, K. Shimokata, N. Ishiguro, and M. Takahashi
Microspherule Protein 1, Mi-2{beta}, and RET Finger Protein Associate in the Nucleolus and Up-regulate Ribosomal Gene Transcription
J. Biol. Chem., November 25, 2005; 280(47): 39436 - 39447.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. C. Valdez, D. Henning, R. B. So, J. Dixon, and M. J. Dixon
The Treacher Collins syndrome (TCOF1) gene product is involved in ribosomal DNA gene transcription by interacting with upstream binding factor
PNAS, July 20, 2004; 101(29): 10709 - 10714.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Halkidou, I. R. Logan, S. Cook, D. E. Neal, and C. N. Robson
Putative involvement of the histone acetyltransferase Tip60 in ribosomal gene transcription
Nucleic Acids Res., March 11, 2004; 32(5): 1654 - 1665.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
I. Grummt
Life on a planet of its own: regulation of RNA polymerase I transcription in the nucleolus
Genes & Dev., July 15, 2003; 17(14): 1691 - 1702.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Hirschler-Laszkiewicz, A. H. Cavanaugh, A. Mirza, M. Lun, Q. Hu, T. Smink, and L. I. Rothblum
Rrn3 Becomes Inactivated in the Process of Ribosomal DNA Transcription
J. Biol. Chem., May 23, 2003; 278(21): 18953 - 18959.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. L. French, Y. N. Osheim, F. Cioci, M. Nomura, and A. L. Beyer
In Exponentially Growing Saccharomyces cerevisiae Cells, rRNA Synthesis Is Determined by the Summed RNA Polymerase I Loading Rate Rather than by the Number of Active Genes
Mol. Cell. Biol., March 1, 2003; 23(5): 1558 - 1568.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. M. Thomas, K. Haleem, A. B. Siddique, W. J. Simmons, N. Sen, D.-J. Zhang, and V. K. Tsiagbe
Regulation of Mouse Mammary Tumor Virus env Transcriptional Activator Initiated Mammary Tumor Virus Superantigen Transcripts in Lymphomas of SJL/J Mice: Role of Ikaros, Demethylation, and Chromatin Structural Change in the Transcriptional Activation of Mammary Tumor Virus Superantigen
J. Immunol., January 1, 2003; 170(1): 218 - 227.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Liu, A. Guo, B. Boukhgalter, K. Van Den Heuvel, M. Tripp, and L. Pape
Characterization of the fission yeast ribosomal DNA binding factor: components share homology with Upstream Activating Factor and with SWI/SNF subunits
Nucleic Acids Res., December 15, 2002; 30(24): 5347 - 5359.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. H. Cavanaugh, I. Hirschler-Laszkiewicz, Q. Hu, M. Dundr, T. Smink, T. Misteli, and L. I. Rothblum
Rrn3 Phosphorylation Is a Regulatory Checkpoint for Ribosome Biogenesis
J. Biol. Chem., July 19, 2002; 277(30): 27423 - 27432.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.