Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (685K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (53)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Pedrazzi, G.
Right arrow Articles by Stagljar, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pedrazzi, G.
Right arrow Articles by Stagljar, I.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2001, Vol. 29, No. 21 4378-4386
© 2001 Oxford University Press

Direct association of Bloom’s syndrome gene product with the human mismatch repair protein MLH1

Graziella Pedrazzi, Claudia Perrera1, Heiko Blaser, Patrick Kuster, Giancarlo Marra1, Sally L. Davies2, Gi-Hyuck Ryu, Raimundo Freire3, Ian D. Hickson2, Josef Jiricny1 and Igor Stagljar*

Institute of Veterinary Biochemistry and Molecular Biology, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland, 1Institute of Medical Radiobiology of the University of Zürich and the Paul Scherrer Institute, August Forel-Strasse 7, CH-8008 Zürich, Switzerland, 2Imperial Cancer Research Fund Laboratiories, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, UK and 3Unidad de Investigacion, Hospital Universitario de Canarias, Ofra s/n, La Cuesta, 38071 Tenerife, Spain

Bloom’s syndrome (BS) is a rare genetic disorder characterised by genomic instability and cancer susceptibility. BLM, the gene mutated in BS, encodes a member of the RecQ family of DNA helicases. Here, we identify hMLH1, which is involved in mismatch repair (MMR) and recombination, as a protein that directly interacts with BLM both in vivo and in vitro, and that the two proteins co-localise to discrete nuclear foci. The interaction between BLM and hMLH1 appears to have been evolutionarily conserved, as Sgs1p, the Saccharomyces cerevisiae homologue of BLM, interacts with yeast Mlh1p. However, cell extracts derived from BS patients show no obvious defects in MMR compared to wild-type- and BLM-complemented BS cell extracts. We conclude that the hMLH1–BLM interaction is not essential for post-replicative MMR, but, more likely, is required for some aspect of genetic recombination.

* To whom correspondence should be addressed. Tel: +41 1 635 54 74; Fax: +41 1 635 68 40; Email: stagljar{at}vetbio.unizh.ch Present address:Gi-Hyuck Ryu, Sungkyunkwan University School of Medicine, 300 Chunchun-Dong, Changan-Ku, Suwon, Kyunggi 440-746, Korea


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
C. Dherin, E. Gueneau, M. Francin, M. Nunez, S. Miron, S. E. Liberti, L. J. Rasmussen, S. Zinn-Justin, B. Gilquin, J.-B. Charbonnier, et al.
Characterization of a Highly Conserved Binding Site of Mlh1 Required for Exonuclease I-Dependent Mismatch Repair
Mol. Cell. Biol., February 1, 2009; 29(3): 907 - 918.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Babbe, J. McMenamin, E. Hobeika, J. Wang, S. J. Rodig, M. Reth, and P. Leder
Genomic Instability Resulting from Blm Deficiency Compromises Development, Maintenance, and Function of the B Cell Lineage
J. Immunol., January 1, 2009; 182(1): 347 - 360.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. Selak, C. Z. Bachrati, I. Shevelev, T. Dietschy, B. van Loon, A. Jacob, U. Hubscher, J. D. Hoheisel, I. D. Hickson, and I. Stagljar
The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase {delta}
Nucleic Acids Res., September 1, 2008; 36(16): 5166 - 5179.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
C. Welz-Voegele and S. Jinks-Robertson
Sequence Divergence Impedes Crossover More Than Noncrossover Events During Mitotic Gap Repair in Yeast
Genetics, July 1, 2008; 179(3): 1251 - 1262.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. Saydam, R. Kanagaraj, T. Dietschy, P. L. Garcia, J. Pena-Diaz, I. Shevelev, I. Stagljar, and P. Janscak
Physical and functional interactions between Werner syndrome helicase and mismatch-repair initiation factors
Nucleic Acids Res., September 27, 2007; 35(17): 5706 - 5716.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. McVey, S. L. Andersen, Y. Broze, and J. Sekelsky
Multiple Functions of Drosophila BLM Helicase in Maintenance of Genome Stability
Genetics, August 1, 2007; 176(4): 1979 - 1992.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Cannavo, B. Gerrits, G. Marra, R. Schlapbach, and J. Jiricny
Characterization of the Interactome of the Human MutL Homologues MLH1, PMS1, and PMS2
J. Biol. Chem., February 2, 2007; 282(5): 2976 - 2986.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
P. E. Cohen, S. E. Pollack, and J. W. Pollard
Genetic Analysis of Chromosome Pairing, Recombination, and Cell Cycle Control during First Meiotic Prophase in Mammals
Endocr. Rev., June 1, 2006; 27(4): 398 - 426.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Petkovic, T. Dietschy, R. Freire, R. Jiao, and I. Stagljar
The human Rothmund-Thomson syndrome gene product, RECQL4, localizes to distinct nuclear foci that coincide with proteins involved in the maintenance of genome stability
J. Cell Sci., September 15, 2005; 118(18): 4261 - 4269.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. M. Doherty, S. Sharma, L. A. Uzdilla, T. M. Wilson, S. Cui, A. Vindigni, and R. M. Brosh Jr.
RECQ1 Helicase Interacts with Human Mismatch Repair Factors That Regulate Genetic Recombination
J. Biol. Chem., July 29, 2005; 280(30): 28085 - 28094.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
R. M. Spell and S. Jinks-Robertson
Examination of the Roles of Sgs1 and Srs2 Helicases in the Enforcement of Recombination Fidelity in Saccharomyces cerevisiae
Genetics, December 1, 2004; 168(4): 1855 - 1865.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. U. Keller-Seitz, U. Certa, C. Sengstag, F. E. Wurgler, M. Sun, and M. Fasullo
Transcriptional Response of Yeast to Aflatoxin B1: Recombinational Repair Involving RAD51 and RAD1
Mol. Biol. Cell, September 1, 2004; 15(9): 4321 - 4336.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Luo, F.-T. Lin, and W.-C. Lin
ATM-Mediated Stabilization of hMutL DNA Mismatch Repair Proteins Augments p53 Activation during DNA Damage
Mol. Cell. Biol., July 15, 2004; 24(14): 6430 - 6444.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
R. Jiao, C. Z. Bachrati, G. Pedrazzi, P. Kuster, M. Petkovic, J.-L. Li, D. Egli, I. D. Hickson, and I. Stagljar
Physical and Functional Interaction between the Bloom's Syndrome Gene Product and the Largest Subunit of Chromatin Assembly Factor 1
Mol. Cell. Biol., June 1, 2004; 24(11): 4710 - 4719.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Cui, D. Arosio, K. M. Doherty, R. M. Brosh Jr, A. Falaschi, and A. Vindigni
Analysis of the unwinding activity of the dimeric RECQ1 helicase in the presence of human replication protein A
Nucleic Acids Res., April 19, 2004; 32(7): 2158 - 2170.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Sharma, J. A. Sommers, L. Wu, V. A. Bohr, I. D. Hickson, and R. M. Brosh Jr.
Stimulation of Flap Endonuclease-1 by the Bloom's Syndrome Protein
J. Biol. Chem., March 12, 2004; 279(11): 9847 - 9856.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
R. M. Spell and S. Jinks-Robertson
Role of Mismatch Repair in the Fidelity of RAD51- and RAD59-Dependent Recombination in Saccharomyces cerevisiae
Genetics, December 1, 2003; 165(4): 1733 - 1744.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. R. Meetei, S. Sechi, M. Wallisch, D. Yang, M. K. Young, H. Joenje, M. E. Hoatlin, and W. Wang
A Multiprotein Nuclear Complex Connects Fanconi Anemia and Bloom Syndrome
Mol. Cell. Biol., May 15, 2003; 23(10): 3417 - 3426.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. Sridharan, M. Brown, W. C. Lambert, L. W. McMahon, and M. W. Lambert
Nonerythroid {alpha}II spectrin is required for recruitment of FANCA and XPF to nuclear foci induced by DNA interstrand cross-links
J. Cell Sci., March 1, 2003; 116(5): 823 - 835.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. L. Argueso, A. W. Kijas, S. Sarin, J. Heck, M. Waase, and E. Alani
Systematic Mutagenesis of the Saccharomyces cerevisiae MLH1 Gene Reveals Distinct Roles for Mlh1p in Meiotic Crossing Over and in Vegetative and Meiotic Mismatch Repair
Mol. Cell. Biol., February 1, 2003; 23(3): 873 - 886.
[Abstract] [Full Text]

Home page
 GeneticsHome page
E. R. Hoffmann, P. V. Shcherbakova, T. A. Kunkel, and R. H. Borts
MLH1 Mutations Differentially Affect Meiotic Functions in Saccharomyces cerevisiae
Genetics, February 1, 2003; 163(2): 515 - 526.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
M. D. Adams, M. McVey, and J. J. Sekelsky
Drosophila BLM in Double-Strand Break Repair by Synthesis-Dependent Strand Annealing
Science, January 10, 2003; 299(5604): 265 - 267.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
A. Franchitto and P. Pichierri
Protecting genomic integrity during DNA replication: correlation between Werner's and Bloom's syndrome gene products and the MRE11 complex
Hum. Mol. Genet., October 1, 2002; 11(20): 2447 - 2453.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Gellon, M. Werner, and S. Boiteux
Ntg2p, a Saccharomyces cerevisiae DNA N-Glycosylase/Apurinic or Apyrimidinic Lyase Involved in Base Excision Repair of Oxidative DNA Damage, Interacts with the DNA Mismatch Repair Protein Mlh1p. IDENTIFICATION OF A Mlh1p BINDING MOTIF
J. Biol. Chem., August 9, 2002; 277(33): 29963 - 29972.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. L. Argueso, D. Smith, J. Yi, M. Waase, S. Sarin, and E. Alani
Analysis of Conditional Mutations in the Saccharomyces cerevisiae MLH1 Gene in Mismatch Repair and in Meiotic Crossing Over
Genetics, March 1, 2002; 160(3): 909 - 921.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.