Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (422K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (10)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Wrzesinski, J.
Right arrow Articles by Ciesiolka, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wrzesinski, J.
Right arrow Articles by Ciesiolka, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2001, Vol. 29, No. 21 4482-4492
© 2001 Oxford University Press

Catalytic cleavage of cis- and trans-acting antigenomic delta ribozymes in the presence of various divalent metal ions

Jan Wrzesinski, Michal Legiewicz, Barbara Smólska and Jerzy Ciesiolka*

Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland

Catalytic activity of four structural variants of the antigenomic delta ribozyme, two cis- and two trans-acting, has been compared in the presence of selected divalent metal ions that effectively support catalysis. The ribozymes differ in regions that are not directly involved in formation of the ribozyme active site: the region immediately preceding the catalytic cleavage site, the P4 stem and a stretch of the viral RNA sequence extending the minimal ribozyme sequence at its 3'-terminus. The variants show high cleavage activity in the presence of Mg2+, Ca2+ and Mn2+, lower with Co2+ and Sr2+ and some variants are also active with Cd2+ and Zn2+ ions. In the presence of a particular metal ion the ribozymes cleave, however with different initial rates, according to pseudo-first or higher order kinetics and to different final cleavage extents. On the other hand, relatively small differences are observed in the reactions induced by various metal ions. The cleavage of trans-acting ribozymes induced by Mg2+ is partially inhibited in the presence of Na+, spermidine and some other divalent metal ions. The inert Co(NH3)63+ complex is unable to support catalysis, as reported earlier for the genomic ribozyme. The results are discussed in terms of the influence of structural elements peripheral to the ribozyme active site on its cleavage rate and efficiency as well as the role of metal ions in the cleavage mechanism. Some implications concerning further studies and possible applications of delta ribozymes are also considered.

* To whom correspondence should be addressed. Tel: +48 61 8528503; Fax: +48 61 8520532; Email: ciesiolk{at}ibch.poznan.pl


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
M. Legiewicz, A. Wichlacz, B. Brzezicha, and J. Ciesiolka
Antigenomic delta ribozyme variants with mutations in the catalytic core obtained by the in vitro selection method
Nucleic Acids Res., March 2, 2006; 34(4): 1270 - 1280.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Dutkiewicz and J. Ciesiolka
Structural characterization of the highly conserved 98-base sequence at the 3' end of HCV RNA genome and the complementary sequence located at the 5' end of the replicative viral strand
Nucleic Acids Res., January 28, 2005; 33(2): 693 - 703.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
J. OUELLET and J.-P. PERREAULT
Cross-linking experiments reveal the presence of novel structural features between a hepatitis delta virus ribozyme and its substrate
RNA, July 1, 2004; 10(7): 1059 - 1072.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Wichlacz, M. Legiewicz, and J. Ciesiolka
Generating in vitro transcripts with homogenous 3' ends using trans-acting antigenomic delta ribozyme
Nucleic Acids Res., February 18, 2004; 32(3): e39 - e39.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.