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Nucleic Acids Research, 2001, Vol. 29, No. 23 4834-4842
© 2001 Oxford University Press

Nearest neighbour analysis of MCM protein complexes in Drosophila melanogaster

Gilles Crevel, Aleks Ivetic, Katsuhito Ohno1, Masamitsu Yamaguchi1 and Sue Cotterill*

Department of Biochemistry, St Georges Hospital Medical School, Cranmer Terrace, London SW17 0RE, UK and 1Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya 464-8681, Japan

The MCM proteins are a group of six proteins whose action is vital for DNA replication in eukaryotes. It has been suggested that they constitute the replicative helicase, with a subset of the proteins forming the catalytic helicase (MCM4,6,7) while the others have a loading or control function. In this paper we show that all six MCM proteins are present in equivalent amounts in soluble extracts and on chromatin. We have also analysed soluble and chromatin-associated MCM protein complexes under different conditions. This suggests that all six MCM proteins are always found in a complex with each other, although the interaction between the individual MCM proteins is not equivalent as stringent salt conditions are able to break the intact complex into a number of stable subcomplexes. These data contribute to the ongoing debate about the nature of MCM complexes, supporting the hypothesis that they act as a heterohexamer rather than as a number of different subcomplexes. Finally, using protein–protein cross-linking we have shown that MCM2 interacts directly with MCM5 and MCM6; MCM5 with MCM3 and MCM2; and MCM6 with MCM2 and MCM4. This provides the first direct information about specific subunit contacts in the MCM complex.

* To whom correspondence should be addressed. Tel: +44 208 725 5751; Fax: +44 208 725 2992; Email: s.cotterill{at}sghms.ac.uk Present addresses:Aleks Ivetic, Division of Cellular Immunology, The National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UKKatshuhito Ohno and Masamitsu Yamaguchi, Division of Biotechnology, Faculty of Textile Sciences, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan


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