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Nucleic Acids Research, 2001, Vol. 29, No. 24 4941-4947
© 2001 Oxford University Press

RNase H mediated cleavage of RNA by cyclohexene nucleic acid (CeNA)

Birgit Verbeure, Eveline Lescrinier, Jing Wang and Piet Herdewijn*

Laboratory of Medicinal Chemistry, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium

Cyclohexene nucleic acid (CeNA) forms a duplex with RNA that is more stable than a DNA–RNA duplex ({Delta}Tm per modification: +2°C). A cyclohexenyl A nucleotide adopts a 3'-endo conformation when introduced in dsDNA. The neighbouring deoxynucleotide adopts an O4'-endo conformation. The CeNA:RNA duplex is cleaved by RNase H. The Vmax and Km of the cleavage reaction for CeNA:RNA and DNA:RNA is in the same range, although the kcat value is about 600 times lower in the case of CeNA:RNA.

* To whom correspondence should be addressed. Tel: +32 16 337387; Fax: +32 16 337340; Email: piet.herdewijn{at}rega.kuleuven.ac.be


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