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Nucleic Acids Research, 2001, Vol. 29, No. 24 5029-5035
© 2001 Oxford University Press

Sequence organization of barley centromeres

Sabina Hudakova, Wolfgang Michalek, Gernot G. Presting, Rogier ten Hoopen, Karla dos Santos, Zuzana Jasencakova and Ingo Schubert*

Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, D-06466 Gatersleben, Germany

By sequencing, fingerprinting and in situ hybridization of a centromere-specific large insert clone (BAC 7), the sequence organization of centromeric DNA of barley could be elucidated. Within 23 kb, three copies of the Ty3/gypsy-like retroelement cereba were present. Two elements of ~7 kb, arranged in tandem, include long terminal repeats (LTRs) (~1 kb) similar to the rice centromeric retrotransposon RIRE 7 and to the cereal centromeric sequence family, the primer binding site, the complete polygene flanked by untranslated regions, as well as a polypurine tract 5' of the downstream LTR. The high density (~200 elements/centromere) and completeness of cereba elements and the absence of internally deleted elements and solo LTRs from the BAC 7 insert represent unique features of the barley centromeres as compared to those of other cereals. Obviously, the conserved cereba elements together with barley-specific G+C-rich satellite sequences constitute the major components of centromeric DNA in this species.

* To whom correspondence should be addressed. Tel: +49 39482 5239; Fax: +49 39482 5137; Email: schubert{at}ipk-gatersleben.de Present addresses:Gernot G. Presting, Novartis Agriculture Discovery Institute, 3115 Marryfield Row, San Diego, CA 92121-U25, USAKarla dos Santos, Institut für Pflanzenbau und Pflanzenzüchtung, Universtät Göttingen, von Siebold Strasse 8, 37075 Göttingen, Germany +AY040832, AY040833


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