Nucleic Acids Research, 2001, Vol. 29, No. 3 598-603
© 2001 Oxford University Press
Stable expression in yeast of the mature form of human telomerase RNA depends on its association with the box H/ACA small nucleolar RNP proteins Cbf5p, Nhp2p and Nop10p
Laboratoire de Biologie Moléculaire Eucaryote du CNRS, 118 route de Narbonne, 31062 Toulouse Cedex 04, France and 1Institute of Cell and Molecular Biology, University of Edinburgh, King’s Buildings, Edinburgh EH9 3JR, UK
Telomerase is a ribonucleoprotein (RNP) particle required for the replication of telomeres. The RNA component, termed hTR, of human telomerase contains a domain structurally and functionally related to box H/ACA small nucleolar RNAs (snoRNAs). Furthermore, hTR is known to be associated with two core components of H/ACA snoRNPs, hGar1p and Dyskerin (the human counterpart of yeast Cbf5p). To assess the functional importance of the association of hTR with H/ACA snoRNP core proteins, we have attempted to express hTR in a genetically tractable system, Saccharomyces cerevisiae. Both mature non-polyadenylated and polyadenylated forms of hTR accumulate in yeast. The former is associated with all yeast H/ACA snoRNP core proteins, unlike TLC1 RNA, the endogenous RNA component of yeast telomerase. We show that the presence of the H/ACA snoRNP proteins Cbf5p, Nhp2p and Nop10p, but not Gar1p, is required for the accumulation of mature non-polyadenylated hTR in yeast, while accumulation of TLC1 RNA is not affected by the absence of any of these proteins. Our results demonstrate that yeast telomerase is unrelated to H/ACA snoRNPs. In addition, they show that the accumulation in yeast of the mature RNA component of human telomerase depends on its association with three of the four core H/ACA snoRNP proteins. It is likely that this is the case in human cells as well.
* To whom correspondence should be addressed. Tel: +33 5 61 33 59 53; Fax: +33 5 61 33 58 86; Email: henry{at}ibcg.biotoul.fr
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Autexier POT of gold: modeling dyskeratosis congenita in the mouse Genes & Dev., July 1, 2008; 22(13): 1731 - 1736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lin, R. Jin, B. Zhang, H. Chen, Y. X. Bai, P. X. Yang, S. W. Han, Y. H. Xie, P. T. Huang, C. Huang, et al. Nucleolar localization of TERT is unrelated to telomerase function in human cells J. Cell Sci., July 1, 2008; 121(13): 2169 - 2176. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Vulliamy, R. Beswick, M. Kirwan, A. Marrone, M. Digweed, A. Walne, and I. Dokal From the Cover: Mutations in the telomerase component NHP2 cause the premature ageing syndrome dyskeratosis congenita PNAS, June 10, 2008; 105(23): 8073 - 8078. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kannan, A. D. L. Nelson, and D. E. Shippen Dyskerin Is a Component of the Arabidopsis Telomerase RNP Required for Telomere Maintenance Mol. Cell. Biol., April 1, 2008; 28(7): 2332 - 2341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Podlevsky, C. J. Bley, R. V. Omana, X. Qi, and J. J.-L. Chen The Telomerase Database Nucleic Acids Res., January 11, 2008; 36(suppl_1): D339 - D343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Marrone, A. Walne, H. Tamary, Y. Masunari, M. Kirwan, R. Beswick, T. Vulliamy, and I. Dokal Telomerase reverse-transcriptase homozygous mutations in autosomal recessive dyskeratosis congenita and Hoyeraal-Hreidarsson syndrome Blood, December 15, 2007; 110(13): 4198 - 4205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Walne, T. Vulliamy, A. Marrone, R. Beswick, M. Kirwan, Y. Masunari, F.-h. Al-Qurashi, M. Aljurf, and I. Dokal Genetic heterogeneity in autosomal recessive dyskeratosis congenita with one subtype due to mutations in the telomerase-associated protein NOP10 Hum. Mol. Genet., July 1, 2007; 16(13): 1619 - 1629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Reichow, T. Hamma, A. R. Ferre-D'Amare, and G. Varani The structure and function of small nucleolar ribonucleoproteins Nucleic Acids Res., March 12, 2007; 35(5): 1452 - 1464. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Kittur, X. Darzacq, S. Roy, R. H. Singer, and U. T. Meier Dynamic association and localization of human H/ACA RNP proteins RNA, December 1, 2006; 12(12): 2057 - 2062. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Normand, R. Capeyrou, S. Quevillon-Cheruel, A. Mougin, Y. Henry, and M. Caizergues-Ferrer Analysis of the binding of the N-terminal conserved domain of yeast Cbf5p to a box H/ACA snoRNA RNA, October 1, 2006; 12(10): 1868 - 1882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Darzacq, N. Kittur, S. Roy, Y. Shav-Tal, R. H. Singer, and U. T. Meier Stepwise RNP assembly at the site of H/ACA RNA transcription in human cells J. Cell Biol., April 24, 2006; 173(2): 207 - 218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Manival, C. Charron, J.-B. Fourmann, F. Godard, B. Charpentier, and C. Branlant Crystal structure determination and site-directed mutagenesis of the Pyrococcus abyssi aCBF5-aNOP10 complex reveal crucial roles of the C-terminal domains of both proteins in H/ACA sRNP activity Nucleic Acids Res., February 2, 2006; 34(3): 826 - 839. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. KHANNA, H. WU, C. JOHANSSON, M. CAIZERGUES-FERRER, and J. FEIGON Structural study of the H/ACA snoRNP components Nop10p and the 3' hairpin of U65 snoRNA. RNA, January 1, 2006; 12(1): 40 - 52. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Lebaron, C. Froment, M. Fromont-Racine, J.-C. Rain, B. Monsarrat, M. Caizergues-Ferrer, and Y. Henry The Splicing ATPase Prp43p Is a Component of Multiple Preribosomal Particles Mol. Cell. Biol., November 1, 2005; 25(21): 9269 - 9282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ballarino, M. Morlando, F. Pagano, A. Fatica, and I. Bozzoni The Cotranscriptional Assembly of snoRNPs Controls the Biosynthesis of H/ACA snoRNAs in Saccharomyces cerevisiae Mol. Cell. Biol., July 1, 2005; 25(13): 5396 - 5403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Dez, C. Froment, J. Noaillac-Depeyre, B. Monsarrat, M. Caizergues-Ferrer, and Y. Henry Npa1p, a Component of Very Early Pre-60S Ribosomal Particles, Associates with a Subset of Small Nucleolar RNPs Required for Peptidyl Transferase Center Modification Mol. Cell. Biol., July 15, 2004; 24(14): 6324 - 6337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bah, F. Bachand, E. Clair, C. Autexier, and R. J. Wellinger Humanized telomeres and an attempt to express a functional human telomerase in yeast Nucleic Acids Res., March 26, 2004; 32(6): 1917 - 1927. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhu, R. L. Tomlinson, A. A. Lukowiak, R. M. Terns, and M. P. Terns Telomerase RNA Accumulates in Cajal Bodies in Human Cancer Cells Mol. Biol. Cell, January 1, 2004; 15(1): 81 - 90. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Lin and M. Momany The Aspergillus nidulans swoC1 Mutant Shows Defects in Growth and Development Genetics, October 1, 2003; 165(2): 543 - 554. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Whitehead, K. W. Jones, X. Zhang, X. Cheng, R. M. Terns, and M. P. Terns Determinants of the Interaction of the Spinal Muscular Atrophy Disease Protein SMN with the Dimethylarginine-modified Box H/ACA Small Nucleolar Ribonucleoprotein GAR1 J. Biol. Chem., December 6, 2002; 277(50): 48087 - 48093. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. K. Yang, G. Rotondo, T. Porras, P. Legrain, and G. Chanfreau The Shq1p{middle dot}Naf1p Complex Is Required for Box H/ACA Small Nucleolar Ribonucleoprotein Particle Biogenesis J. Biol. Chem., November 15, 2002; 277(47): 45235 - 45242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Dez, J. Noaillac-Depeyre, M. Caizergues-Ferrer, and Y. Henry Naf1p, an Essential Nucleoplasmic Factor Specifically Required for Accumulation of Box H/ACA Small Nucleolar RNPs Mol. Cell. Biol., October 15, 2002; 22(20): 7053 - 7065. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-S. Cong, W. E. Wright, and J. W. Shay Human Telomerase and Its Regulation Microbiol. Mol. Biol. Rev., September 1, 2002; 66(3): 407 - 425. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. T. Etheridge, S. S. R. Banik, B. N. Armbruster, Y. Zhu, R. M. Terns, M. P. Terns, and C. M. Counter The Nucleolar Localization Domain of the Catalytic Subunit of Human Telomerase J. Biol. Chem., June 28, 2002; 277(27): 24764 - 24770. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Mergny, J.-F. Riou, P. Mailliet, M.-P. Teulade-Fichou, and E. Gilson Natural and pharmacological regulation of telomerase Nucleic Acids Res., February 15, 2002; 30(4): 839 - 865. [Abstract] [Full Text] [PDF]
|
|