Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (382K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kamali-Moghaddam, M.
Right arrow Articles by Sundström, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kamali-Moghaddam, M.
Right arrow Articles by Sundström, L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2001, Vol. 29, No. 4 1005-1011
© 2001 Oxford University Press

Arrayed transposase-binding sequences on the ends of transposon Tn5090/Tn402

Masood Kamali-Moghaddam and Lars Sundström*

Department of Pharmaceutical Biosciences, Division of Microbiology, Uppsala University, PO Box 581 BMC, S-751 23 Uppsala, Sweden

The transposon Tn5090/Tn402 encodes a 559 amino acid transposase, TniA, with a DDE motif. Gel mobility shifting and cleavage protection analysis with DNase I and hydroxyl radical probes revealed that TniA binds to multiple repeat sequences on either terminus of Tn5090/Tn402. Four of these TniA-binding 19mers occurred on the left-hand (t) end and two on the right-hand (i) end. Hydroxyl radical cleavage protection demonstrated the presence of 3–6 bp contact sequences on one face of the DNA helix. The binding pattern and organisation of repeats suggested parallels between Tn5090/Tn402 and Mu, which controls its transpositional activity in the assembly step of a higher order transpososome complex. The complex terminal structure and genes of transposase and nucleotide-binding proteins in tandem are hallmarks of the handful of Mu-like elements that are known to date.

* To whom correspondence should be addressed. Tel: +46 18 4714115; Fax: +46 18 502790; Email: lars.sundstrom{at}farmbio.uu.se


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
R. S. Levings, S. P. Djordjevic, and R. M. Hall
SGI2, a Relative of Salmonella Genomic Island SGI1 with an Independent Origin
Antimicrob. Agents Chemother., July 1, 2008; 52(7): 2529 - 2537.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
T. Sugiyama, T. Iida, K. Izutsu, K.-S. Park, and T. Honda
Precise Region and the Character of the Pathogenicity Island in Clinical Vibrio parahaemolyticus Strains
J. Bacteriol., March 1, 2008; 190(5): 1835 - 1837.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
H. W. Stokes, C. L. Nesbo, M. Holley, M. I. Bahl, M. R. Gillings, and Y. Boucher
Class 1 integrons potentially predating the association with tn402-like transposition genes are present in a sediment microbial community.
J. Bacteriol., August 1, 2006; 188(16): 5722 - 5730.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
M. L. Riccio, L. Pallecchi, J.-D. Docquier, S. Cresti, M. R. Catania, L. Pagani, C. Lagatolla, G. Cornaglia, R. Fontana, and G. M. Rossolini
Clonal Relatedness and Conserved Integron Structures in Epidemiologically Unrelated Pseudomonas aeruginosa Strains Producing the VIM-1 Metallo-{beta}-Lactamase from Different Italian Hospitals
Antimicrob. Agents Chemother., January 1, 2005; 49(1): 104 - 110.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
C. M. Collis, M.-J. Kim, S. R. Partridge, H. W. Stokes, and R. M. Hall
Characterization of the Class 3 Integron and the Site-Specific Recombination System It Determines
J. Bacteriol., June 1, 2002; 184(11): 3017 - 3026.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.