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Nucleic Acids Research, 2001, Vol. 29, No. 5 1080-1086
© 2001 Oxford University Press

Functionality of the STNV translational enhancer domain correlates with affinity for two wheat germ factors

Rosalinde van Lipzig, Marc Van Montagu, Marc Cornelissen1 and Frank Meulewaeter1,*

Vakgroep Moleculaire Genetica, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium and 1Aventis CropScience N.V., J. Plateaustraat 22, B-9000 Gent, Belgium

The satellite tobacco necrosis virus RNA is uncapped and requires a 3' translational enhancer domain (TED) for translation. Both in the wheat germ extract and in tobacco, TED stimulates in cis translation of heterologous, uncapped RNAs. In this study we investigated to what extent translation stimulation by TED depends on binding to wheat germ factors. We show that in vitro TED binds at least seven wheat germ proteins. Translation and crosslinking assays, to which TED or TED derivatives with reduced functionality were included as competitor, showed that TED function correlates with binding to a 28 kDa protein (p28). One particular condition of competition revealed that p28 binding is not obligatory for TED function. Under this condition, a 30 kDa protein (p30) binds to TED. Importantly, affinity of p30 correlates with functionality of TED. These results strongly suggest that TED has the capacity to stimulate translation by recruiting the translational machinery either via binding to p28 or via binding to p30.

* To whom correspondence should be addressed. Tel: +32 9 235 84 38; Fax: +32 9 224 06 94; Email: frank.meulewaeter{at}aventis.com


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