Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (355K) Freely available
Right arrow Supplementary Data
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (31)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Jose, A. M.
Right arrow Articles by Breaker, R. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jose, A. M.
Right arrow Articles by Breaker, R. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2001, Vol. 29, No. 7 1631-1637
© 2001 Oxford University Press

Cooperative binding of effectors by an allosteric ribozyme

Antony M. Jose, Garrett A. Soukup and Ronald R. Breaker*

Department of Molecular, Cellular and Developmental Biology, KBT 452, Yale University, PO Box 208103, New Haven, CT 06520-8103, USA

An allosteric ribozyme that requires two different effectors to induce catalysis was created using modular rational design. This ribozyme construct comprises five conjoined RNA modules that operate in concert as an obligate FMN- and theophylline-dependent molecular switch. When both effectors are present, this ‘binary’ RNA switch self-cleaves with a rate enhancement of ~300-fold over the rate observed in the absence of effectors. Kinetic and structural studies implicate a switching mechanism wherein FMN binding induces formation of the active ribozyme conformation. However, the binding site for FMN is rendered inactive unless theophylline first binds to its corresponding site and reorganizes the RNA structure. This example of cooperative binding between allosteric effectors reveals a level of structural and functional complexity for RNA that is similar to that observed with allosteric proteins.

* To whom correspondence should be addressed. Tel: +1 203 432 9389; Fax: +1 203 432 5713; Email: ronald.breaker{at}yale.edu Present address: Garrett A. Soukup, Department of Biomedical Sciences, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J. J. Tabor, M. Levy, and A. D. Ellington
Deoxyribozymes that recode sequence information.
Nucleic Acids Res., January 1, 2006; 34(8): 2166 - 2172.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
M. Mandal, M. Lee, J. E. Barrick, Z. Weinberg, G. M. Emilsson, W. L. Ruzzo, and R. R. Breaker
A Glycine-Dependent Riboswitch That Uses Cooperative Binding to Control Gene Expression
Science, October 8, 2004; 306(5694): 275 - 279.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. H. Najafi-Shoushtari, G. Mayer, and M. Famulok
Sensing complex regulatory networks by conformationally controlled hairpin ribozymes
Nucleic Acids Res., June 15, 2004; 32(10): 3212 - 3219.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Ferguson, R. M. Boomer, M. Kurz, S. C. Keene, J. L. Diener, A. D. Keefe, C. Wilson, and S. T. Cload
A novel strategy for selection of allosteric ribozymes yields RiboReporterTM sensors for caffeine and aspartame
Nucleic Acids Res., March 16, 2004; 32(5): 1756 - 1766.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
S. K. SILVERMAN
Rube Goldberg goes (ribo)nuclear? Molecular switches and sensors made from RNA
RNA, April 1, 2003; 9(4): 377 - 383.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. Y. Wang, B. H. Y. Lai, A. R. Feldman, and D. Sen
A general approach for the use of oligonucleotide effectors to regulate the catalysis of RNA-cleaving ribozymes and DNAzymes
Nucleic Acids Res., April 15, 2002; 30(8): 1735 - 1742.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.