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Nucleic Acids Research, 2002, Vol. 30, No. 11 2261-2269
© 2002 Oxford University Press

Replicational organization of three weakly expressed loci in Physarum polycephalum

Chrystelle Maric, Emma Swanston1, Juliet Bailey1 and Gérard Pierron*

Institut André Lwoff, UPR-1983, Laboratoire Réplication et Ultrastructure du Noyau, 7 rue Guy Moquet, F-94801-Villejuif, France and 1Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK

We previously mapped early-activated replication origins in the promoter regions of five abundantly transcribed genes in the slime mold Physarum polycephalum. This physical linkage between origins and genes is congruent with the preferential early replication of the active genes in mammalian cells. To determine how general this replicational organization is in the synchronous plasmodium of Physarum, we analyzed the replication of three weakly expressed genes. Bromodeoxyuridine (BrdUrd) density-shift and gene dosage experiments indicated that the redB (regulated in development) and redE genes replicate early, whereas redA replicates in mid-S phase. Bi-dimensional gel electrophoresis revealed that redA coincides with an origin that appears to be activated within a large temporal window in S phase so that the replication of the gene is not well defined temporally. The early replication of the redB and redE genes is due to the simultaneous activation of flanking origins at the onset of S phase. As a result, these two genes correspond to termination sites of DNA replication. Our data demonstrate that not all the Physarum promoters are preferred sites of initiation but, so far, all the expressed genes analyzed in detail either coincide with a replication origin or are embedded into a cluster of early firing replicons.

* To whom correspondence should be addressed. Tel: +33 1 49 58 33 73; Fax: +33 1 49 58 33 81; Email: pierron{at}infobiogen.fr Present address: Juliet Bailey, Research and Business Development Office, University of Leicester, University Road, Leicester LE1 7RH, UK The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors


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M. Benard, C. Maric, and G. Pierron
Low rate of replication fork progression lengthens the replication timing of a locus containing an early firing origin
Nucleic Acids Res., September 27, 2007; 35(17): 5763 - 5774.
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