Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (455K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (21)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Georgakilas, A. G.
Right arrow Articles by Sutherland, B. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Georgakilas, A. G.
Right arrow Articles by Sutherland, B. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2002, Vol. 30, No. 13 2800-2808
© 2002 Oxford University Press

High efficiency detection of bi-stranded abasic clusters in {gamma}-irradiated DNA by putrescine

Alexandros G. Georgakilas, Paula V. Bennett and Betsy M. Sutherland*

Biology Department, Building 463, Brookhaven National Laboratory, Upton, NY 11973-5000, USA

Bi-stranded abasic clusters, an abasic (AP) site on one DNA strand and another nearby AP site or strand break on the other, have been quantified using Nfo protein from Escherichia coli to produce a double-strand break at cluster sites. Since recent data suggest that Nfo protein cleaves inefficiently at some clusters, we tested whether polyamines, which also cut at AP sites, would cleave abasic clusters at higher efficiency. The data show that Nfo protein cleaves poorly at clusters containing immediately opposed AP sites and those separated by 1 or 3 bp. Putrescine (PUTR) cleaved more efficiently than spermidine or spermine, and did not cleave undamaged DNA. It cleaved abasic clusters in oligonucleotide duplexes more effectively than Nfo protein, including immediately opposed or closely spaced clusters. PUTR cleaved more efficiently than Nfo protein by a factor of ~1.7 or ~2 for DNA that had been {gamma}-irradiated in moderate or non-radioquenching conditions, respectively. This suggests that the DNA environment during irradiation affects the spectrum of cluster configurations. Further comparison of PUTR and Nfo protein cleavage may provide useful information on abasic cluster levels and configurations induced by ionizing radiation.

* To whom correspondence should be addressed. Tel: +1 631 344 3380; Fax: +1 631 344 3407; Email: bms{at}bnl.gov


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
S. G. Kozmin, Y. Sedletska, A. Reynaud-Angelin, D. Gasparutto, and E. Sage
The formation of double-strand breaks at multiply damaged sites is driven by the kinetics of excision/incision at base damage in eukaryotic cells
Nucleic Acids Res., April 1, 2009; 37(6): 1767 - 1777.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
B. Paap, D. M. Wilson III, and B. M. Sutherland
Human abasic endonuclease action on multilesion abasic clusters: implications for radiation-induced biological damage
Nucleic Acids Res., May 1, 2008; 36(8): 2717 - 2727.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
P. D. Chastain II, J. Nakamura, J. Swenberg, and D. Kaufman
Nonrandom AP site distribution in highly proliferative cells
FASEB J, December 1, 2006; 20(14): 2612 - 2614.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Datta, R. D. Neumann, and T. A. Winters
Characterization of complex apurinic/apyrimidinic-site clustering associated with an authentic site-specific radiation-induced DNA double-strand break
PNAS, July 26, 2005; 102(30): 10569 - 10574.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. G. Georgakilas, P. V. Bennett, D. M. Wilson III, and B. M. Sutherland
Processing of bistranded abasic DNA clusters in {gamma}-irradiated human hematopoietic cells
Nucleic Acids Res., October 19, 2004; 32(18): 5609 - 5620.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.