Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (273K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Bane, T. K.
Right arrow Articles by Riggs, A. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bane, T. K.
Right arrow Articles by Riggs, A. D.
Related Collections
Right arrow Protein-nucleic acid interaction
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2002, Vol. 30, No. 14 e69
© 2002 Oxford University Press

DNA affinity capture and protein profiling by SELDI-TOF mass spectrometry: effect of DNA methylation

Thomas K. Bane, James F. LeBlanc2, Terry D. Lee1 and Arthur D. Riggs*

Division of Biology and 1 Division of Immunology, Beckman Research Institute, The City of Hope National Medical Center, 1450 East Duarte Road, Duarte, CA 91010, USA and 2 Ciphergen Biosystems, Fremont, CA 94555, USA

*To whom correspondence should be addressed. Tel: +1 626 359 8111; Fax: +1 626 930 5366; Email: ariggs{at}coh.org

We report here that surface enhanced laser desorption/ionization–time of flight (SELDI-TOF) mass spectrometry, as performed on a Ciphergen Biosystems ProteinChip System, can be used in conjunction with DNA affinity capture (DACA) to study specific DNA–protein binding. Using DNA molecules bound to a surface, sequence-specific interactions can be detected as demonstrated by a mutation affecting the binding profile for TBP, a transcription factor. Also, a comparison between methylated and unmethylated promoter-containing DNA fragments shows numerous binding profile differences over a mass range extending to >60 kDa. The binding of several proteins is inhibited by methylation of the DNA.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
N. Shanmugam, M. A. Reddy, and R. Natarajan
Distinct Roles of Heterogeneous Nuclear Ribonuclear Protein K and microRNA-16 in Cyclooxygenase-2 RNA Stability Induced by S100b, a Ligand of the Receptor for Advanced Glycation End Products
J. Biol. Chem., December 26, 2008; 283(52): 36221 - 36233.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
D. Harris, Z. Zhang, B. Chaubey, and V. N. Pandey
Identification of Cellular Factors Associated with the 3'-Nontranslated Region of the Hepatitis C Virus Genome
Mol. Cell. Proteomics, June 1, 2006; 5(6): 1006 - 1018.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.