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Nucleic Acids Research, 2002, Vol. 30, No. 19 e98
© 2002 Oxford University Press

PfoI, a unique type II restriction endonuclease that recognises the sequence 5'-T{downarrow}CCNGGA-3'

M. Gaigalas, Z. Maneliene, R. Kazlauskiene1, M. Petrusyte and A. Janulaitis*

Institute of Biotechnology and 1 Fermentas UAB, Graiciuno 8, 2028 Vilnius, Lithuania

*To whom correspondence should be addressed. Tel: +370 2 602110; Fax: +370 2 602116; Email: janulait{at}ibt.lt

A new type II restriction endonuclease designated PfoI has been partially purified from Pseudomonas fluorescens biovar 126. PfoI recognises the interrupted hexanucleotide palindromic sequence 5'-T{downarrow}CCNGGA-3' and cleaves DNA to produce protruding pentanucleotide 5'-ends.


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